Development of Colloidal Gold-Based Immunochromatographic Strips for Rapid Detection and Surveillance of Japanese Encephalitis Virus in Dogs across Shanghai, China
Author:
Zhong Dengke1, Wahaab Abdul23ORCID, Zheng Jiayang2, Zhang Junjie2, Ma Zhiyong2, Wei Jianchao2ORCID
Affiliation:
1. Shanghai Vocational College of Agriculture and Forestry, Shanghai 201600, China 2. Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China 3. Department of Entomology, Center for Infectious Disease Dynamics and The Huck Institutes of the Life Sciences, Pennsylvania State University, University Park, PA 16801, USA
Abstract
Japanese encephalitis virus (JEV) causes acute encephalitis in humans and is of major public health concern in most Asian regions. Dogs are suitable sentinels for assessing the risk of JEV infection in humans. A neutralization test (NT) or an enzyme-linked immunosorbent assay (ELISA) is used for the serological detection of JEV in dogs; however, these tests have several limitations, and, thus, a more convenient and reliable alternative test is needed. In this study, a colloidal gold immunochromatographic strip (ICS), using a purified recombinant EDIII protein, was established for the serological survey of JEV infection in dogs. The results show that the ICSs could specifically detect JEV antibodies within 10 min without cross-reactions with antibodies against other canine viruses. The test strips could detect anti-JEV in serum with dilution up to 640 times, showing high sensitivity. The coincidence rate with the NT test was higher than 96.6%. Among 586 serum samples from dogs in Shanghai examined using the ICS test, 179 (29.98%) were found to be positive for JEV antibodies, and the high seropositivity of JEV in dogs in China was significantly correlated with the season and living environment. In summary, we developed an accurate and economical ICS for the rapid detection of anti-JEV in dog serum samples with great potential for the surveillance of JEV in dogs.
Funder
Shanghai Agriculture Applied Technology Development Program, China Animal Biosecurity Prevention and Control in Lancang Mekong Countries Shanghai Municipal Science and Technology Major Project Agricultural Science and Technology Innovation Program
Subject
Virology,Infectious Diseases
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