Characterization of a Ligilactobacillus salivarius Strain Isolated from a Cheese Seal Which Was Last Used in 1936

Author:

Alba Claudio1ORCID,Arroyo Rebeca1,Fernández Leónides2ORCID,Narbad Arjan3,Rodríguez Juan M.1ORCID

Affiliation:

1. Department of Nutrition and Food Science, Complutense University of Madrid, 28040 Madrid, Spain

2. Department of Galenic Pharmacy and Food Technology, Complutense University of Madrid, 28040 Madrid, Spain

3. Food Microbiome and Health Institute Strategic Programme, Quadram Institute Bioscience, Rosalind Franklin Road, Colney, Norwich NR4 7UQ, UK

Abstract

Cheesemaking played a pivotal role in the life of the Pyrenean villages where cheese was a most prized commodity and the subject of much local competition. In one of them (Sasa de Sobrepuerto), Mrs. Sebastiana Palacio decided in 1877 to label all the cheeses made in her household with a seal to differentiate them from those made by other local producers. The cheese seal was last used in 1936 and, since then, it has been kept under excellent storage conditions. Since well-preserved cheese seals are rare, and bacterial cells may survive desiccation for long periods, the objective of this work was to isolate and characterize any lactic acid bacteria that survived in the seal. Analysis of the milky crust material revealed the presence of sheep caseins. Culture-based analysis led to the isolation of a strain of Bacillus licheniformis and a strain of Ligilactobacillus salivarius (L. salivarius SP36). The latter was characterized in vitro for safety and dairy-related functional properties. Its genome encodes several genes involved in protein, peptide, and amino acid catabolism, and flavor. Overall, the phenotypic and genetic features of this strain support a high potential for being used as adjunct culture in cheesemaking.

Publisher

MDPI AG

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