Molecular Characterization of Listeria monocytogenes in the Food Chain of the Republic of Kosovo from 2016 to 2022-Reference-Cited by-同舟云学术

Molecular Characterization of Listeria monocytogenes in the Food Chain of the Republic of Kosovo from 2016 to 2022

Published:2024-09-12 Issue:18 Volume:13 Page:2883
ISSN:2304-8158
Container-title:Foods
language:en
Short-container-title:Foods

Author:

Jashari Besart¹,Capitaine Karine²^ORCID,Bisha Bledar³^ORCID,Stessl Beatrix⁴^ORCID,Blagoevska Katerina⁵^ORCID,Cana Armend⁶^ORCID,Jankuloski Dean⁵^ORCID,Félix Benjamin²^ORCID

Affiliation:

1. Food Microbiology, Food and Veterinary Laboratory, Food and Veterinary Agency of Kosovo, Lidhja e Pejës 241, 10000 Pristina, Kosovo

2. Laboratory for Food Safety, Salmonella and Listeria Unit, ANSES, European Union Reference Laboratory for L. monocytogenes, University of Paris-Est, 94700 Maisons-Alfort, France

3. Department of Animal Science, College of Agriculture and Natural Resources, University of Wyoming, Laramie, WY 82071, USA

4. Unit of Food Microbiology, Centre for Food Science and Public Veterinary Health, Clinical Department for Farm Animals and Food Systems Safety, University of Veterinary Medicine Vienna, Veterinärplatz 1, A-2110 Vienna, Austria

5. Food Institute, Faculty of Veterinary Medicine—Skopje, Ss. Cyril and Methodius University in Skopje, Lazar Pop-Trajkov 5–7, 1000 Skopje, North Macedonia

6. Microbiology Laboratory, University for Business and Technology—Higher Education Institution, Kalabria, 10000 Pristina, Kosovo

Abstract

The present study describes the genetic characterization of L. monocytogenes strains found in the Republic of Kosovo’s food chain. From 2016 to 2022, 995 samples were collected. Overall, 648 samples were from ready-to-eat (RTE) food products, 281 from food products consumed cooked (FPCC), 60 from raw materials, and 6 from environmental samples. Overall, 11.76% (117 out of 995) of the samples were contaminated by L. monocytogenes, comprising 6.33% (41 out of 648) from RTE products, 14.95% (42 out of 281) from FPCC, 55.00% (33 out of 60) from raw materials, and 16.66% (1 out of 6) from environmental samples. All isolates were subjected to molecular serotyping and clonal complex (CC) identification by using real-time PCR, as well as multilocus sequence typing. All isolates were grouped into four molecular serotypes, IIa (34.19%), IIb (3.48%), IIc (32.48%), and IVb (29.91%), as well as Lineage I (33.33%) and Lineage II (66.66%). In total, 14 CCs were identified from 41 RTE isolates; however, CC29 (7), CC2 (6), and CC6 (6) were the most dominant. By contrast, CC9 was by far the most represented CC in both FPCC (21) and RM (14). Moreover, 30 isolates expressed CC1, CC2, CC4, or CC6, which are particularly associated with severe human infections.

Publisher

MDPI AG

Link

https://www.mdpi.com/2304-8158/13/18/2883/pdf

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