Optimizing Chlorella vulgaris Cultivation to Enhance Biomass and Lutein Production
Author:
Wu Kangping12, Lai Jiangling1, Zhang Qi1ORCID, Wang Yunpu1, Cui Xian1, Liu Yuhuan3ORCID, Wu Xiaodan1, Yu Zhigang4ORCID, Ruan Roger5ORCID
Affiliation:
1. State Key Laboratory of Food Science and Resources, Engineering Research Center for Biomass Conversion, Ministry of Education, Nanchang University, Nanchang 330047, China 2. School of Resources and Civil Engineering, Gannan University of Science and Technology, Ganzhou 341000, China 3. College of Food Science and Technology, Nanchang University, Nanchang 330047, China 4. Australian Centre for Water and Environmental Biotechnology (Formerly AWMC), The University of Queensland, Brisbane, QLD 4072, Australia 5. Center for Biorefining and Department of Bioproducts and Biosystems Engineering, University of Minnesota, St. Paul, MN 55108, USA
Abstract
Lutein is widely used in medicine, health care, and food processing due to its antioxidant effects; however, it is difficult for the traditional extraction of lutein using marigolds to meet the increasing market demand for lutein. To achieve high-efficiency lutein production, we investigated the effects of different conditions on the biomass accumulation and lutein yield of Chlorella vulgaris. The optimized cultivation conditions include mixotrophic cultivation using sodium nitrate as a nitrogen source, maintaining a total-organic-carbon-to-total-nitrogen ratio of 12:1, a total-nitrogen-to-total-phosphorus ratio of 10:1, and lighting duration of 24 h. The results of the study indicated that under these specific conditions, Chlorella vulgaris attained a final biomass concentration, biomass productivity, and growth yield of 6.08 g·L−1, 1.00 g·L−1·d−1, and 1.67 g biomass/g TOC, respectively. Additionally, the concentrations of total chlorophyll, carotenoid, lutein, and protein reached 139.20 mg·L−1, 31.87 mg·L−1, 15.02 mg·L−1, and 2.17 g·L−1, respectively, and the content of lutein reached 2.47 mg·g−1. This study supplies a theoretical basis for the industrial application of lutein production using Chlorella vulgaris.
Funder
National Natural Science Foundation of China Major Discipline Academic and Technical Leaders Training Program of Jiangxi Province Natural Science Foundation of Jiangxi Province Natural Science Foundation of Chongqing City Research Project of the State Key Laboratory of Food Science and Resource of Nanchang University
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