Differences in Extracellular NAD+ and NMN Metabolism on the Surface of Vascular Endothelial Cells

Author:

Jablonska PatrycjaORCID,Mierzejewska Paulina,Tomczyk MartaORCID,Koszalka Patrycja,Franczak MarikaORCID,Kawecka Ada,Kutryb-Zajac BarbaraORCID,Braczko AlicjaORCID,Smolenski Ryszard T.ORCID,Slominska Ewa M.ORCID

Abstract

The disruption of the metabolism of extracellular NAD+ and NMN may affect related signaling cascades and pathologies, such as cardiovascular or respiratory system diseases. We aimed to study NAD+ and NMN hydrolysis on surface endothelial cells of diverse origins and with genetically modified nucleotide catabolism pathways. We tested lung endothelial cells isolated from C57BL/6 J wild-type (WT) and C57BL/6 J CD73 knockout (CD73 KO) mice, the transfected porcine iliac artery endothelial cell line (PIEC) with the human E5NT gene for CD73 (PIEC CD73), and a mock-transfected control (PIEC MOCK), as well as HMEC-1 and H5V cells. Substrate conversion into the product was followed by high-performance liquid chromatography (HPLC). We showed profound differences in extracellular NAD+ and NMN metabolism related to the vessel origin, species diversity, and type of culture. We also confirmed the involvement of CD38 and CD73 in NAD+ and NMN cleavage.

Funder

Polish Ministry of Science and Higher Education

Publisher

MDPI AG

Subject

General Agricultural and Biological Sciences,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology

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