Climate and Wolbachia Impacts on Anoplolepis gracilipes (Hymenoptera: Formicidae)

Abstract

The yellow crazy ant (Anoplolepis gracilipes (Smith, 1857)) is a prominent invasive species exhibiting variable population dynamics. Through collecting long-term climate data and validating field surveys with MaxEnt SDM projections, our results indicated that winter temperature and precipitation accumulation strongly influence the population dynamics. An aggression analysis showed that A. gracilipes nests with higher aggression levels (over 2.5 scores) experienced a higher mean maximum temperature (31.84 ± 0.43 °C) and lower prevalence of wAgra (84.8 ± 4.70%) in A. gracilipes from June to October. The nest manipulation and aggression experiments confirmed that temperature increases aggression (1.3 to 2.8 scores) among A. gracilipes workers due to the reduced prevalence of wAgra. To the best of our knowledge, this is the first case of a notable reduction in the prevalence of Wolbachia (100 to 66%) within a colony of A. gracilipes while maintaining stable nests for further experiments.