Toward a Platform for the Treatment of Burns: An Assessment of Nanoemulsions vs. Nanostructured Lipid Carriers Loaded with Curcumin
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Published:2023-12-18
Issue:12
Volume:11
Page:3348
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ISSN:2227-9059
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Container-title:Biomedicines
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language:en
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Short-container-title:Biomedicines
Author:
Araújo Gabriela de Moraes Soares1, Loureiro Ana Isabel Sá2, Rodrigues Jamile Lima3, Barros Paula Alice Bezerra1, Halicki Priscila Cristina Bartolomeu1, Ramos Daniela Fernandes1ORCID, Marinho Marcelo Augusto Germani4, Vaiss Daniela Pastorim1, Vaz Gustavo Richter1ORCID, Yurgel Virginia Campello1, Bidone Juliana5, Muccillo-Baisch Ana Luiza1, Hort Mariana Appel1ORCID, Paulo Artur Manuel Cavaco2ORCID, Dora Cristiana Lima13ORCID
Affiliation:
1. Graduate Program in Health Sciences, Federal University of Rio Grande, Rio Grande 96203-900, RS, Brazil 2. CEB—Center of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal 3. Graduate Program in Food Science and Engineering, Federal University of Rio Grande, Rio Grande 96203-900, RS, Brazil 4. Graduate Program in Physiology, Federal University of Rio Grande, Rio Grande 96203-900, RS, Brazil 5. Center of Chemical, Pharmaceutical, and Food Sciences, Federal University of Pelotas, Pelotas 96010-610, RS, Brazil
Abstract
Curcumin is a highly promising substance for treating burns, owing to its anti-inflammatory, antioxidant, antimicrobial, and wound-healing properties. However, its therapeutic use is restricted due to its hydrophobic nature and low bioavailability. This study was conducted to address these limitations; it developed and tested two types of lipid nanocarriers, namely nanoemulsions (NE-CUR) and nanostructured lipid carriers (NLC-CUR) loaded with curcumin, and aimed to identify the most suitable nanocarrier for skin burn treatment. The study evaluated various parameters, including physicochemical characteristics, stability, encapsulation efficiency, release, skin permeation, retention, cell viability, and antimicrobial activity. The results showed that both nanocarriers showed adequate size (~200 nm), polydispersity index (~0.25), and zeta potential (~>−20 mV). They also showed good encapsulation efficiency (>90%) and remained stable for 120 days at different temperatures. In the release test, NE-CUR and NCL-CUR released 57.14% and 51.64% of curcumin, respectively, in 72 h. NE-CUR demonstrated better cutaneous permeation/retention in intact or scalded skin epidermis and dermis than NLC-CUR. The cell viability test showed no toxicity after treatment with NE-CUR and NLC-CUR up to 125 μg/mL. Regarding microbial activity assays, free curcumin has activity against P. aeruginosa, reducing bacterial growth by 75% in 3 h. NE-CUR inhibited bacterial growth by 65% after 24 h, and the association with gentamicin had favorable results, while NLC-CUR showed a lower inhibition. The results demonstrated that NE-CUR is probably the most promising nanocarrier for treating burns.
Funder
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior–Brazil Portuguese Foundation for Science and Technology
Subject
General Biochemistry, Genetics and Molecular Biology,Medicine (miscellaneous)
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