The Value of CXCL1, CXCL2, CXCL3, and CXCL8 as Potential Prognosis Markers in Cervical Cancer: Evidence of E6/E7 from HPV16 and 18 in Chemokines Regulation
-
Published:2023-09-28
Issue:10
Volume:11
Page:2655
-
ISSN:2227-9059
-
Container-title:Biomedicines
-
language:en
-
Short-container-title:Biomedicines
Author:
Fernandez-Avila Leonardo12ORCID, Castro-Amaya Aribert Maryosly2ORCID, Molina-Pineda Andrea34ORCID, Hernández-Gutiérrez Rodolfo3ORCID, Jave-Suarez Luis Felipe12ORCID, Aguilar-Lemarroy Adriana12ORCID
Affiliation:
1. Programa de Doctorado en Ciencias Biomédicas, Centro Universitario de Ciencias de la Salud (CUCS), Universidad de Guadalajara, Guadalajara 44340, Jalisco, Mexico 2. División de Inmunología, Centro de Investigación Biomédica de Occidente (CIBO), Instituto Mexicano del Seguro Social (IMSS), Guadalajara 44340, Jalisco, Mexico 3. Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, A.C., Guadalajara 44270, Jalisco, Mexico 4. Consejo Nacional de Ciencia y Tecnología, CONAHCYT, Mexico City 03940, Mexico
Abstract
Cervical cancer (CC) is a serious global health issue, and it is well-known that HPV infection is the main etiological factor that triggers carcinogenesis. In cancer, chemokine ligands and receptors are involved in tumor cell growth, metastasis, leukocyte infiltration, and angiogenesis; however, information on the role played by E6/E7 of HPV16/18 in the modulation of chemokines is very limited. Therefore, this study aimed to determine whether chemokines are differentially expressed in CC-derived cell lines; if E6/E7 oncoproteins from HPV16 and 18 are capable of mediating chemokine expression, what is the expression profile of chemokines in tissues derived from CC and what is their impact on the overall survival of patients with this pathology? For this purpose, RNA sequencing and real-time PCR were performed on SiHa, HeLa, and C33A tumorigenic cell lines, on the non-tumorigenic HaCaT cells, and the E6/E7 HPV-transduced HaCaT cell models. Furthermore, chemokine expression and survival analysis were executed on 304 CC and 22 normal tissue samples from The Cancer Genome Atlas (TCGA) repository. The results demonstrate that CXCL1, CXCL2, CXCL3, and CXCL8 are regulated by E6/E7 of HPV16 and 18, are overexpressed in CC biopsies, and that their higher expression is related to a worse prognostic survival.
Funder
Fondo de Investigación en Salud (FIS)—Instituto Mexicano del Seguro Social
Subject
General Biochemistry, Genetics and Molecular Biology,Medicine (miscellaneous)
Reference90 articles.
1. 2020 WHO Classification of Female Genital Tumors;Brambs;Geburtshilfe Frauenheilkd,2021 2. Global estimates of incidence and mortality of cervical cancer in 2020: A baseline analysis of the WHO Global Cervical Cancer Elimination Initiative;Singh;Lancet Glob. Health,2023 3. Cervical cancer in low and middle-income countries (Review);Hull;Oncol. Lett.,2020 4. Bruni, L.A.G., Serrano, B., Mena, M., Gómez, D., Muñoz, J., Bosch, F.X., and de Sanjosé, S. (2019). Human Papillomavirus and Related Diseases in Mexico, Institut Català d’Oncologia/IARC. Summary Report. 5. Prendiville, W.S.R. (2017). Colposcopy and Treatment of Cervical Precancer, World Health Organization.
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献
|
|