Gut Fungal Microbiota Alterations in Pulmonary Arterial Hypertensive Rats
-
Published:2024-01-27
Issue:2
Volume:12
Page:298
-
ISSN:2227-9059
-
Container-title:Biomedicines
-
language:en
-
Short-container-title:Biomedicines
Author:
Chen Yihang12, Meng Liukun3, Yuan Wen4, Gao Zehan5, Zhang Xun6ORCID, Xie Boqia12, Song Jiawei12, Li Jifeng5ORCID, Zhong Jiuchang12ORCID, Liu Xiaoyan124ORCID
Affiliation:
1. Department of Cardiology, Beijing Chao-Yang Hospital, Capital Medical University, Beijing 100020, China 2. Heart Center and Beijing Key Laboratory of Hypertension, Beijing Institute of Respiratory Medicine, Beijing Chao-Yang Hospital, Capital Medical University, Beijing 100020, China 3. State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Disease, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100032, China 4. Medical Research Center, Beijing Institute of Respiratory Medicine and Beijing Chao-Yang Hospital, Capital Medical University, Beijing 100020, China 5. Department of Respiratory and Critical Care Medicine, Beijing Institute of Respiratory Medicine and Beijing Chao-Yang Hospital, Capital Medical University, Beijing 100020, China 6. Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
Abstract
The gut microbiome’s imbalance has been implicated in the pathogenesis of pulmonary arterial hypertension (PAH), yet the contribution of the gut mycobiome remains largely unclear. This study delineates the gut mycobiome profile in PAH and examines its interplay with the bacterial microbiome alterations. Fecal samples from monocrotaline-induced PAH rats and matched controls were subjected to internal transcribed spacer 1 (ITS1) sequencing for fungal community assessment and 16S ribosomal RNA (rRNA) gene sequencing for bacterial community characterization. Comparative analysis revealed no significant disparities in the overall mycobiome diversity between the PAH and control groups. However, taxonomic profiling identified differential mycobiome compositions, with the PAH group exhibiting a significant enrichment of genera such as Wallemia, unidentified_Branch02, Postia, Malassezia, Epicoccum, Cercospora, and Alternaria. Conversely, genera Xeromyces, unidentified_Plectosphaerellaceae, and Monilia were more abundant in the controls. Correlations of Malassezia and Wallemia abundance with hemodynamic parameters were observed. Indications of bidirectional fungal–bacterial community interactions were also noted. This investigation reveals distinct gut mycobiome alterations in PAH, which are intricately associated with concurrent bacterial microbiome changes, suggesting a possible contributory role of gut fungi in PAH pathophysiology. These findings underscore the potential for novel gut mycobiome-targeted therapeutic interventions in PAH management.
Funder
National Natural Science Foundation of China National Key Clinical Specialty Construction Project - Cardiovascular Surgery, the Beijing Hospitals Authority Youth Program Natural Science Foundation of Beijing Clinical Research Incubation Program of Beijing Chaoyang Hospital Affiliated to Capital Medical University 2024 Reform and Development Program of Beijing Institute of Respiratory Medicine
Subject
General Biochemistry, Genetics and Molecular Biology,Medicine (miscellaneous)
Reference29 articles.
1. Altered gut microbiome profile in patients with pulmonary arterial hypertension;Kim;Hypertension,2020 2. Jiang, S., Shui, Y., Cui, Y., Tang, C., Wang, X., Qiu, X., Hu, W., Fei, L., Li, Y., and Zhang, S. (2021). Gut microbiota dependent trimethylamine N-oxide aggravates angiotensin II–induced hypertension. Redox Biol., 46. 3. Karoor, V., Strassheim, D., Sullivan, T., Verin, A., Umapathy, N.S., Dempsey, E.C., Frank, D.N., Stenmark, K.R., and Gerasimovskaya, E. (2021). The short-chain fatty acid butyrate attenuates pulmonary vascular remodeling and inflammation in hypoxia-induced pul-monary hypertension. Int. J. Mol. Sci., 22. 4. A human gut microbial gene catalogue established by metagenomic sequencing;Qin;Nature,2010 5. The mycobiota: Interactions between commensal fungi and the host immune system;Underhill;Nat. Rev. Immunol.,2014
|
|