Solid Lipid Nanoparticles from Platonia insignis Seeds, a Brazilian Amazon Fruit: Characterization, In Vitro and In Vivo Toxicological and Antioxidant Activities
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Published:2023-09-01
Issue:9
Volume:7
Page:368
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ISSN:2504-477X
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Container-title:Journal of Composites Science
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language:en
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Short-container-title:J. Compos. Sci.
Author:
Coêlho Angélica Gomes1, de Almeida José Otávio Carvalho Sena1, Santos Andressa Amorim dos1, Santos Webysten Ronny Pereira dos1, da Rocha Sousa Leonardo1, Viana Nildomar Ribeiro1, Batista Felipe Alves2, de Sousa Brito Neta Maria3, Santos Alexandre Silva3ORCID, da Silva Sebastião William3ORCID, de Souza Paulo Eduardo Narcizo3, Rodrigues de Araújo-Nobre Alyne4, de Sousa Lima-Neto José5, das Graças Lopes Citó Antônia Maria6, de Amorim Carvalho Fernando Aécio7, de Moraes Alves Michel Muálem7, Lucarini Massimo8ORCID, Durazzo Alessandra8ORCID, Nogueira Mendes Anderson2ORCID, Arcanjo Daniel Dias Rufino1ORCID
Affiliation:
1. LAFMOL–Laboratory of Functional and Molecular Studies in Physiopharmacology, Department of Biophysics and Physiology, Federal University of Piauí, Teresina 64049-550, Brazil 2. LACITEC–Laboratory of Innovation on Science and Technology, Department of Biophysics and Physiology, Federal University of Piauí, Teresina 64049-550, Brazil 3. Institute of Physics, University of Brasília, Brasília 70919-970, Brazil 4. Núcleo de Pesquisa em Biodiversidade e Biotecnologia, Biotec, Campus Ministro Reis Velloso, Universidade Federal do Delta do Parnaíba, Parnaíba 64202-020, Brazil 5. Faculty of Pharmacy, Federal University of Piauí, Teresina 64049-550, Brazil 6. LAGO–Laboratory of Organic Geochemistry, Federal University of Piauí, Teresina 64049-550, Brazil 7. Medicinal Plants Research Center, Federal University of Piauí, Teresina 64049-550, Brazil 8. CREA–Research Centre for Food and Nutrition, Via Ardeatina 546, 00178 Rome, Italy
Abstract
Solid Lipid Nanoparticles (SLNs) are drug delivery systems with important advantages over conventional nanosystems. Considering previously reported pharmacological and physicochemical properties of Platonia insignis seed butter (BBI), this work aimed at developing, characterizing and performing toxicological and antioxidant studies of SLNs produced from BBI. The GC-MS analysis identified palmitic and oleic acids as the major compounds. Three SLN prototypes were developed through high-shear homogenization followed by ultrasonication. During a 180-day stability evaluation, the formulation SLN/TW-1.5 presented greater stability since pH was around 6.0, as well as a lesser variation of the PdI (Polydispersity Index), particle size and Zeta Potential (ZP), confirmed with Raman Spectroscopy and Atomic Force Microscopy (AFM). The CC50 in macrophages was around 249.4 µg∙mL−1 for BBI, whereas the CC50 value for SLN/TW-1.5 was 45.2 µg∙mL−1. Electron Paramagnetic Resonance (EPR) showed a marked in vitro antioxidant activity for BBI and SLN/TW-1.5. After in vivo SLN/TW-1.5 administration in Zophobas morio larvae, assessment of reduced glutathione (GSH), nitrite (NO2−) and myeloperoxidase (MPO) demonstrated antioxidant activity. Thus, the intrinsic physicochemical properties of BBI allowed the development of an optimized nanoformulation with high stability indexes, besides the great potential for antioxidant applications.
Subject
Engineering (miscellaneous),Ceramics and Composites
Reference115 articles.
1. Editorial: The State of Science and Innovation of Bioactive Research and Applications, Health, and Diseases;Durazzo;Front. Nutr.,2019 2. Souto, E.B., Silva, G.F., Dias-Ferreira, J., Zielinska, A., Ventura, F., Durazzo, A., Lucarini, M., Novellino, E., and Santini, A. (2020). Nanopharmaceutics: Part I-Clinical Trials Legislation and Good Manufacturing Practices (GMP) of Nanotherapeutics in the EU. Pharmaceutics, 12. 3. Souto, E.B., Silva, G.F., Dias-Ferreira, J., Zielinska, A., Ventura, F., Durazzo, A., Lucarini, M., Novellino, E., and Santini, A. (2020). Nanopharmaceutics: Part II-Production Scales and Clinically Compliant Production Methods. Nanomaterials, 10. 4. Blanco-Llamero, C., Fonseca, J., Durazzo, A., Lucarini, M., Santini, A., Señoráns, F.J., and Souto, E.B. (2022). Nutraceuticals and Food-Grade Lipid Nanoparticles: From Natural Sources to a Circular Bioeconomy Approach. Foods, 11. 5. Solid Lipid Nanoparticles and Nanostructured Lipid Carriers: Structure, Preparation and Application;Naseri;Adv. Pharm. Bull.,2015
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