Molecular Identification of Piroplasmids in Ticks from Infested Small Ruminants in Konya Province, Turkey
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Published:2023-09-01
Issue:9
Volume:12
Page:1123
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ISSN:2076-0817
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Container-title:Pathogens
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language:en
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Short-container-title:Pathogens
Author:
Ma Zhuowei1, Ceylan Onur2ORCID, Galon Eloiza May3ORCID, Mohanta Uday Kumar14ORCID, Ji Shengwei1, Li Hang1, Do Thanh Thom1, Umemiya-Shirafuji Rika1ORCID, El-Sayed Shimaa Abd El-Salam15ORCID, Zafar Iqra1, Liu Mingming6, Sevinc Ferda2ORCID, Xuan Xuenan1ORCID
Affiliation:
1. National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, Japan 2. Department of Parasitology, Faculty of Veterinary Medicine, Selcuk University, 42250 Konya, Turkey 3. College of Veterinary Medicine and Biomedical Sciences, Cavite State University, Indang 4122, Philippines 4. Department of Microbiology and Parasitology, Sher-e-Bangla Agricultural University, Sher-e-Bangla Nagar, Dhaka 1207, Bangladesh 5. Department of Biochemistry and Chemistry of Nutrition, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt 6. School of Basic Medicine, Hubei University of Arts and Science, Xiangyang 441053, China
Abstract
Ticks play a pivotal role in propagating a diverse spectrum of infectious agents that detrimentally affect the health of both humans and animals. In the present study, a molecular survey was executed of piroplasmids in ticks collected from small ruminants in four districts within Konya province, Turkey. Microscopic examination identified 1281 adult ticks, which were categorized into 357 pools based on their species, sexes, host animals, and collection site before DNA extraction. The infection rates were calculated by using a maximum likelihood estimate (MLE) with 95% confidence intervals (CI). Hyalomma detritum, H. excavatum, Rhipicephalus bursa, R. sanguineus, and R. turanicus were identified in this study. Among the five tick species identified here, R. turanicus exhibited the highest infestation rate in both goats and sheep. The presence of Babesia ovis and Theileria ovis based on 18S rRNA was confirmed using molecular assay. The overall MLE of infection rates for B. ovis and T. ovis was 2.49% (CI 1.72–3.46) and 1.46% (CI 0.87–2.23), respectively. The MLE of B. ovis and T. ovis infection rates in R. bursa was 10.80% (CI 7.43–14.90) and 0.33% (CI 0.02–1.42), respectively, while that in R. turanicus was 0.12% (CI 0.01–0.51) and 2.08% (CI 1.25–3.22). This study further confirms that R. turanicus and R. sanguineus can act as vectors for B. ovis, thus advancing our comprehension of tick-borne piroplasmids epidemiology and providing valuable insights for the development of effective control strategies for ticks and tick-borne diseases in Turkey.
Funder
Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan JSPS Core-to-Core Program, Japan
Subject
Infectious Diseases,Microbiology (medical),General Immunology and Microbiology,Molecular Biology,Immunology and Allergy
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