Altered Proteomic Profile of Exosomes Secreted from Vero Cells Infected with Porcine Epidemic Diarrhea Virus
Author:
Shen Xuehuai123ORCID, Yin Lei12, Xu Shuangshuang12, Wang Jieru12ORCID, Yin Dongdong12, Zhao Ruihong12, Pan Xiaocheng12, Dai Yin12, Hou Hongyan12, Zhou Xueli12, Hu Xiaomiao12
Affiliation:
1. Livestock and Poultry Epidemic Diseases Research Center of Anhui Province, Institute of Animal Husbandry and Veterinary Science, Anhui Academy of Agricultural Science, Hefei 230031, China 2. Anhui Province Key Laboratory of Livestock and Poultry Product Safety Engineering, Hefei 230031, China 3. College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China
Abstract
Porcine epidemic diarrhea virus (PEDV) infection causes severe diarrhea in pigs and can be fatal in newborn piglets. Exosomes are extracellular vesicles secreted by cells that transfer biologically active proteins, lipids, and RNA to neighboring or distant cells. Herein, the morphology, particle size, and secretion of exosomes derived from a control and PEDV-infected group are examined, followed by a proteomic analysis of the exosomes. The results show that the exosomes secreted from the Vero cells had a typical cup–shaped structure. The average particle size of the exosomes from the PEDV-infected group was 112.4 nm, whereas that from the control group was 150.8 nm. The exosome density analysis and characteristic protein determination revealed that the content of exosomes in the PEDV-infected group was significantly higher than that in the control group. The quantitative proteomics assays revealed 544 differentially expressed proteins (DEPs) in the PEDV-infected group’s exosomes compared with those in the controls, with 236 upregulated and 308 downregulated proteins. The DEPs were closely associated with cellular regulatory pathways, such as the phosphatidylinositol–4,5–bisphosphate 3–kinase (PI3K)–protein kinase B (Akt) signaling pathway, extracellular matrix–receptor interaction, focal adhesion, and cytoskeletal regulation. These findings provide the basis for further investigation of the pathogenic mechanisms of PEDV and the discovery of novel antiviral targets.
Funder
Key Research and development program of Anhui Province Special Fund for Anhui Agriculture Research System National Natural Science Foundation of China
Subject
Virology,Infectious Diseases
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