The Circulating miRNA Profile of Chronic Hepatitis D and B Patients Is Comparable but Differs from That of Individuals with HBeAg-Negative HBV Infection-Reference-Cited by-同舟云学术

The Circulating miRNA Profile of Chronic Hepatitis D and B Patients Is Comparable but Differs from That of Individuals with HBeAg-Negative HBV Infection

Published:2023-11-15 Issue:11 Volume:15 Page:2257
ISSN:1999-4915
Container-title:Viruses
language:en
Short-container-title:Viruses

Author:

Cavallone Daniela¹²,Ornos Eric¹³⁴^ORCID,Ricco Gabriele¹^ORCID,Oliveri Filippo¹^ORCID,Coco Barbara¹^ORCID,Colombatto Piero¹,De Rosa Laura⁵⁶^ORCID,Dalmacio Leslie⁷,Bonino Ferruccio¹²^ORCID,Brunetto Maurizia¹²⁸^ORCID

Affiliation:

1. Hepatology Unit and Laboratory of Molecular Genetics and Pathology of Hepatitis Viruses, Reference Centre of the Tuscany Region for Chronic Liver Disease and Cancer, University Hospital of Pisa, Via Paradisa 2, 56124 Pisa, Italy

2. Institute of Biostructure and Bioimaging, National Research Council, Via De Amicis 95, 80145 Naples, Italy

3. Department of Medical Microbiology, College of Medicine, University of the Philippines Manila, Pedro Gil Street, Ermita, Manila 1000, Philippines

4. Fondazione Italiana Fegato (FIF), 34149 Trieste, Italy

5. Institute of Clinical Physiology, National Research Council, 56124 Pisa, Italy

6. Department of Information Engineering and Computer Science, University of Trento, 38123 Trento, Italy

7. Department of Biochemistry and Molecular Biology, College of Medicine, University of the Philippines Manila, Pedro Gil Street, Ermita, Manila 1000, Philippines

8. Department of Clinical and Experimental Medicine, University of Pisa, 56126 Pisa, Italy

Abstract

miRNAs circulating in whole serum and HBsAg-particles are differentially expressed in chronic hepatitis B (CHB) and HBeAg-negative-HBV infection (ENI); their profiles are unknown in chronic hepatitis D (CHD). Serum- and HBsAg-associated miRNAs were analyzed in 75 subjects of 3 well-characterized groups (CHB 25, CHD 25, ENI 25) using next-generation sequencing (NGS). Overall miRNA profiles were consonant in serum and HBsAg-particles but significantly different according to the presence of hepatitis independently of Hepatitis D Virus (HDV)-co-infection. Stringent (Bonferroni Correction < 0.001) differential expression analysis showed 39 miRNAs upregulated in CHB vs. ENI and 31 of them also in CHD vs. ENI. miRNA profiles were coincident in CHB and CHD with only miR-200a-3p upregulated in CHB. Three miRNAs (miR-625-3p, miR-142-5p, and miR-223-3p) involved in immune response were upregulated in ENI. All 3 hepatocellular miRNAs of MiR-B-Index (miR-122-5p, miR-99a-5p, miR-192-5p) were overexpressed in both CHB and CHD patients. In conclusion, CHD and CHB patients showed highly similar serum miRNA profiling that was significantly different from that of individuals with HBeAg-negative infection and without liver disease.

Funder

Hepatology Unit Research Fund

Publisher

MDPI AG

Subject

Virology,Infectious Diseases

Link

https://www.mdpi.com/1999-4915/15/11/2257/pdf

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