Molecular Cloning and Expression Analysis of Geranyllinalool Synthase Gene (SgGES) from Salvia guaranitica Plants-Reference-Cited by-同舟云学术

Molecular Cloning and Expression Analysis of Geranyllinalool Synthase Gene (SgGES) from Salvia guaranitica Plants

Published:2024-06-24 Issue:7 Volume:10 Page:668
ISSN:2311-7524
Container-title:Horticulturae
language:en
Short-container-title:Horticulturae

Author:

Abdelhameed Ahmed Ali¹^ORCID,Eissa Mohamed A.²³^ORCID,El-kholy Ragab I.⁴,Darwish Doaa Bahaa Eldin⁵^ORCID,Abeed Amany H. A.⁶^ORCID,Soudy Fathia A.⁷^ORCID,Alyamani Amal Ahmed⁸^ORCID,Abdelmigid Hala M.⁸^ORCID,Morsi Maissa M.⁹,Zhao Jian¹⁰¹¹^ORCID,Ali Mohammed¹²^ORCID,Zayed Muhammad¹³^ORCID

Affiliation:

1. Agricultural Botany Department (Genetics), Faculty of Agriculture, Al-Azhar University, Assuit Branch, Assuit 71524, Egypt

2. Biotechnology Department, Faculty of Agriculture, Al-Azhar University, Cairo 11754, Egypt

3. Zhejiang BioAsia Institute of Life Sciences, Pinghu 314200, China

4. Agricultural Botany (Genetics) Department, Faculty of Agriculture, Al-Azhar University, Cairo 11884, Egypt

5. Biology Department, Faculty of Science, University of Tabuk, Tabuk 71491, Saudi Arabia

6. Department of Botany & Microbiology, Faculty of Science, Assiut University, Assiut 71516, Egypt

7. Genetics and Genetic Engineering Department, Faculty of Agriculture, Benha University, Moshtohor 13736, Egypt

8. Department of Biotechnology, College of Science, Taif University, Taif 21944, Saudi Arabia

9. Department of Biology, College of Science, Taif University, Taif 21944, Saudi Arabia

10. Key Laboratory of Tea Science of Ministry of Education, College of Horticulture, Hunan Agricultural University, Changsha 410128, China

11. National Research Center of Engineering and Technology for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha 410128, China

12. Maryout Research Station, Genetic Resources Department, Desert Research Center, 1 Mathaf El-Matarya St., El-Matareya, Cairo 11753, Egypt

13. Department of Botany and Microbiology, Faculty of Science, Menoufia University, Shebin El-Kom 32511, Egypt

Abstract

Salvia guaranitica is considered one of the most significant medicinal and aromatic herbs in terms of nutritional and medical benefits due to its wealth of important active components. Among these compounds, terpenoids are the most prominent and abundant, particularly monoterpenes (C10), sesquiterpenes (C15), and diterpenes (C20). They are biologically advantageous to plants and perform a multitude of functions. The current study aimed to clone the S. guaranitica gene that encodes for geranyllinalool synthases (SgGES, EC: 4.2.3.144), with consideration for these features. The open reading frame of the 867-amino-acid protein encoded by SgGES consists of 2.721 base pairs. In addition, the SgGES protein has five domains that belong to the terpene synthase family, which are related to the terpene and terpenoid synthase domains. We manipulated and overexpressed the SgGES gene in Nicotiana tabacum to explore its function. When compared to the GUS control, the transgenic N. tabacum plants displayed an increase in leaf production and diameter when compared with the wild-type plants. Finally, analysis of transgenic plants using gas chromatography/mass spectrometry (GC-MS) showed that SgGES is responsible for producing various terpene species, especially diterpenes.

Funder

Taif University, Saudi Arabia

Publisher

MDPI AG

Link

https://www.mdpi.com/2311-7524/10/7/668/pdf

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