Morphological and Molecular Characterization of the Potato Rot Nematode, Ditylenchus destructor, Parasitizing Garlic in Korea-Reference-Cited by-同舟云学术

Morphological and Molecular Characterization of the Potato Rot Nematode, Ditylenchus destructor, Parasitizing Garlic in Korea

Published:2024-08-26 Issue:9 Volume:10 Page:902
ISSN:2311-7524
Container-title:Horticulturae
language:en
Short-container-title:Horticulturae

Author:

Huh Sungchan¹,Park Sohee²,Je Hwanseok³,Park Namsook⁴,Kim Donggeun⁴,Choi Insoo¹⁴,Kang Heonil¹⁴^ORCID

Affiliation:

1. Department of Plant Bioscience, Pusan National University, Miryang 50463, Republic of Korea

2. Division of Crop Protection, National Institute of Agricultural Science, Rural Development Administration, Wanju 55365, Republic of Korea

3. Warm Temperate and Subtropical Forest Research Center, National Institute of Forest Science, Jeju 63582, Republic of Korea

4. Nematode Research Center, Life and Industry Convergence Research Institute, Pusan National University, Miryang 50463, Republic of Korea

Abstract

A survey of plant parasitic nematodes was carried out in 650 garlic fields in Korea from 2020 to 2022. Migratory endoparasite nematodes (Ditylenchus sp.) were recovered from 6% of the garlic samples, with an average density of 494 individuals per garlic bulb. The morphological characteristics of males and females from the 2022 survey were very similar to D. destructor, but D. dipsaci was not found. The Korean population traits have a lateral field containing six incisures, and the posterior esophagus part overlaps the intestine dorsally. PCR and DNA sequencing were performed for the D2/D3 region of the ribosomal DNA 28S and the ITS region, and the phylogenetic analysis strongly supports the monophyly of D. destructor. This is the first report of D. destructor parasitizing garlic in the Republic of Korea. In Korea, due to changes in agricultural or environmental conditions, the most damaging potential PPNs changed from D. dipsaci to D. destructor in garlic cultivation.

Funder

Cooperative Research Program for Agriculture Science and Technology Department

Publisher

MDPI AG

Link

https://www.mdpi.com/2311-7524/10/9/902/pdf

Reference40 articles.

1. (2019). Major Statistics of Agriculture, Livestock and Food, Ministry of Agriculture, Food and Rural Affairs. Available online: https://lib.mafra.go.kr/skyblueimage/28195.pdf.

2. (2022). Survey of Results of Garlic and Onion Cultivation Area, Statistics Korea. Available online: https://kostat.go.kr/boardDownload.es?bid=229&list_no=417923&seq=3.

3. The use of Caenorhabditis elegans as a model for plant-parasitic nematodes: What have we learned?;Coke;Annu. Rev. Phytopathol.,2024

4. Perry, R.N., and Moens, M. (2006). Migratory endoparasitic nematodes. Plant Nematology, CAB International.

5. Ditylenchus laurae sp. n. (Tylenchida: Anguinidae) from Poland—A new species of the D. dipsaci complex associated with a water plant, Potamogeton perfoliatus L.;Skwiercz;Nematology,2017