Construction of a Tomato (Solanum lycopersicum L.) Introgression Line Population and Mapping of Major Agronomic Quantitative Trait Loci

Author:

Chen Yifan12,Qiu Shuliang2,Zhou Hui2,Gao Wenzheng2,Cui Lipeng2,Qiu Zhuoyao2,Dong Chenchen2,Wang Xiaoxuan2

Affiliation:

1. College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China

2. Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China

Abstract

Tomato as a fresh fruit has a large market share in China, but few new materials have been developed for such cultivar breeding in recent years. This study aims to create innovative breeding materials for fresh fruit tomatoes with consistent genetic backgrounds and take advantage of beneficial genes from wild germplasm resources. An introgression line (IL) population was constructed using freshly cultivated tomato S. lycopersicum 1052 and wild tomato S. pennellii LA0716 through hybridization and five consecutive backcrossings, with molecular marker-assisted selection techniques during seedling stages. A total of 447 cleaved amplified polymorphic sequences (CAPS) and 525 simple sequence repeat (SSR) markers were used to screen polymorphic markers among the two parental lines, resulting in 216 polymorphic CAPS and 236 polymorphic SSR markers, with 46.5% parental polymorphism. Then, 200 molecular markers uniformly distributed over the entire genome were further selected, and 107 ILs were finally obtained from 541 BC5 candidate plants. The physical distance between adjacent markers was 6.3~10.0 cm, with an average interval of 7.29 cm, and the IL population constructed covered the whole genome of S. pennillii LA0716, with an average introgression segment of 31.5 cm. Moreover, phenotype data of major agronomic traits in BC5 progeny after selfing two times, were analyzed for quantitative trait locus (QTL) mapping, and a total of 11 QTLs distributed on 6 chromosomes were identified, including 3 QTLs regulating plant height, 1 QTL regulating leaf size, 1 QTL regulating fruit color, 4 QTLs regulating fruit weight, and 2 QTLs regulating soluble solids content in ripening fruits. The IL population constructed in this study provided good materials for fresh fruit tomato breeding with improved yield and quality in the future.

Funder

Hainan Special PhD Scientific Research Foundation of Sanya Yazhou Bay Science and Technology City

China Agriculture Research System

National Natural Science Foundation of China

Publisher

MDPI AG

Subject

Horticulture,Plant Science

Reference32 articles.

1. Taxonomy of wild tomatoes and their relatives (Solanum sections lycopersicoides, Juglandilolia, Lycopersicon; Solanaceae);Peralta;Syst. Bot. Monogr.,2008

2. The breeding of crop ideotypes;Donald;Euphytica,1968

3. Qiu, S.L. (2012). Construction of Solanum Pennellii LA0716, Introgression Lines and Mapping of Related Agronomic Traits. [Master’s Thesis, Chinese Academy of Agricultural Sciences].

4. Trait discovery and editing in tomato;Rothan;Plant J.,2019

5. RFLP mapping in plant breeding: New tools for an old science;Tanksley;Nat. Biotechnol.,1989

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3