Affiliation:
1. Department of Biological Sciences and BK21 Team for Field-Oriented BioCore Human Resources Development, Kongju National University, 56 Gongjudaehak-ro, Gongju 32588, Republic of Korea
Abstract
Cymbidium goeringii Reichb. fil., locally known as the spring orchid in the Republic of Korea, is one of the most important and popular horticultural species in the family Orchidaceae. C. goeringii cultivars originated from plants with rare phenotypes in wild mountains where pine trees commonly grow. This study aimed to determine the cultivar-specific combined genotypes (CGs) of short sequence repeats (SSRs) by analyzing multiple samples per cultivar of C. goeringii. In this study, we collected more than 4000 samples from 67 cultivars and determined the genotypes of 12 SSRs. Based on the most frequent combined genotypes (CG1s), the average observed allele number and combined matching probability were 11.8 per marker and 3.118 × 10−11, respectively. Frequencies of the CG1 in 50 cultivars (n ≥ 10) ranged from 40.9% to 100.0%, with an average of 70.1%. Assuming that individuals with the CG1 are genuine in the corresponding cultivars, approximately 30% of C. goeringii on the farms and markets may be not genuine. The dendrogram of the phylogenetic tree and principal coordinate analysis largely divided the cultivars into three groups according to their countries of origin; however, the genetic distances were not great among the cultivars. In conclusion, this dataset of C. goeringii cultivar-specific SSR profiles could be used for ecogenetic studies and forensic authentication. This study suggests that genetic authentication should be introduced for the sale of expensive C. goeringii cultivars. We believe that this study will help establish a genetic method for the forensic authentication of C. goeringii cultivars.
Funder
National Research Foundation in the Republic of Korea
Subject
Genetics (clinical),Genetics
Reference28 articles.
1. DuPuy, D., and Cribb, P.J. (2007). The Genus Cymbidium, Kew Publishing.
2. Ethnobotany of Orchidaceae-a manual;Arditti;Orchid Biology: Reviews and Perspectives,1994
3. Genetic authentication of cultivars with flower-variant types using SSR markers in spring orchid, Cymbidium goeringii;Lee;Hortic. Environ. Biotechnol.,2020
4. Analysis of phenotypic and genetic polymorphism of self-pollinated seedlings of Korean native Cymbidium goeringii;Lee;Korean J. Hortic. Sci. Technol.,2004
5. Autonomous self-pollination and insect visitors in partially and fully mycoheterotrophic species of Cymbidium (Orchidaceae);Suetsugu;J. Plant Res.,2015