Quantitative Trait Locus Analysis of Hessian Fly Resistance in Soft Red Winter Wheat-Reference-Cited by-同舟云学术

Quantitative Trait Locus Analysis of Hessian Fly Resistance in Soft Red Winter Wheat

Published:2023-09-17 Issue:9 Volume:14 Page:1812
ISSN:2073-4425
Container-title:Genes
language:en
Short-container-title:Genes

Author:

Bagwell John W.¹^ORCID,Subedi Madhav¹^ORCID,Sapkota Suraj²,Lopez Benjamin³,Ghimire Bikash¹⁴^ORCID,Chen Zhenbang³,Buntin G. David⁵,Bahri Bochra A.¹⁴,Mergoum Mohamed¹³

Affiliation:

1. Institute of Plant Breeding, Genetics and Genomics, University of Georgia, Griffin Campus, Griffin, GA 30223, USA

2. Small Grains and Potato Germplasm Research Unit, United States Department of Agriculture Agricultural Research Service, Aberdeen, ID 83210, USA

3. Department of Crop and Soil Sciences, University of Georgia, Griffin Campus, Griffin, GA 30223, USA

4. Department of Plant Pathology, University of Georgia, Griffin Campus, Griffin, GA 30223, USA

5. Department of Entomology, University of Georgia, Griffin Campus, Griffin, GA 30223, USA

Abstract

The Hessian fly (HF) is an invasive insect that has caused millions of dollars in yield losses to southeastern US wheat farms. Genetic resistance is the most sustainable solution to control HF. However, emerging biotypes are quickly overcoming resistance genes in the southeast; therefore, identifying novel sources of resistance is critical. The resistant line “UGA 111729” and susceptible variety “AGS 2038” were crossbred to generate a population of 225 recombinant inbred lines. This population was phenotyped in the growth chamber (GC) during 2019 and 2021 and in field (F) trials in Georgia during the 2021–2022 growing seasons. Visual scoring was utilized in GC studies. The percentage of infested tillers and number of pupae/larvae per tiller, and infested tiller per sample were measured in studies from 2021 to 2022. Averaging across all traits, a major QTL on chromosome 3D explained 42.27% (GC) and 10.43% (F) phenotypic variance within 9.86 centimorgans (cM). SNP marker IWB65911 was associated with the quantitative trait locus (QTL) peak with logarithm of odds (LOD) values of 14.98 (F) and 62.22 (GC). IWB65911 colocalized with resistance gene H32. KASP marker validation verified that UGA 111729 and KS89WGRC06 express H32. IWB65911 may be used for marker-assisted selection.

Funder

University of Georgia Institute of Plant Breeding, Genetics and Genomics, the state of Georgia, and USDA National Institute of Food and Agriculture Hatch Project

Publisher

MDPI AG

Subject

Genetics (clinical),Genetics

Link

https://www.mdpi.com/2073-4425/14/9/1812/pdf

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