A Comparative Proteomic Analysis to Explore the Influencing Factors on Endometritis Using LC-MS/MS

Author:

Jiang Xingcan12,Li Ziyuan12,Chang Xiyv12,Lian Zhengjie12,Wang Aihua13,Lin Pengfei12ORCID,Chen Huatao12ORCID,Zhou Dong12,Tang Keqiong12,Jin Yaping12

Affiliation:

1. Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, College of Veterinary Medicine, Northwest A&F University, Yangling, Xianyang 712100, China

2. Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling, Xianyang 712100, China

3. Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling, Xianyang 712100, China

Abstract

The inflammatory system activated by uterine infection is associated with decreased fertility. Diseases can be detected in advance by identifying biomarkers of several uterine diseases. Escherichia coli is one of the most frequent bacteria that is involved in pathogenic processes in dairy goats. The purpose of this study was to investigate the effect of endotoxin on protein expression in goat endometrial epithelial cells. In this study, the LC–MS/MS approach was employed to investigate the proteome profile of goat endometrial epithelial cells. A total of 1180 proteins were identified in the goat Endometrial Epithelial Cells and LPS-treated goat Endometrial Epithelial Cell groups, of which, 313 differentially expressed proteins were accurately screened. The proteomic results were independently verified by WB, TEM and IF techniques, and the same conclusion was obtained. To conclude, this model is suitable for the further study of infertility caused by endometrial damage caused by endotoxin. These findings may provide useful information for the prevention and treatment of endometritis.

Funder

Research and Application of Prevention and Control Technology for Reproductive Disorders in Dairy cows

National Natural Science Foundation of China

Publisher

MDPI AG

Subject

Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis

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