Combining the Peptide RWQWRWQWR and an Ethanolic Extract of Bidens pilosa Enhances the Activity against Sensitive and Resistant Candida albicans and C. auris Strains-Reference-Cited by-同舟云学术

Combining the Peptide RWQWRWQWR and an Ethanolic Extract of Bidens pilosa Enhances the Activity against Sensitive and Resistant Candida albicans and C. auris Strains

Published:2023-08-02 Issue:8 Volume:9 Page:817
ISSN:2309-608X
Container-title:Journal of Fungi
language:en
Short-container-title:JoF

Author:

Vargas-Casanova Yerly¹^ORCID,Bravo-Chaucanés Claudia¹^ORCID,Martínez Andrea²^ORCID,Costa Geison²^ORCID,Contreras-Herrera Jorge³,Medina Ricardo⁴,Rivera-Monroy Zuly⁴^ORCID,García-Castañeda Javier⁴^ORCID,Parra-Giraldo Claudia¹^ORCID

Affiliation:

1. Microbiology Department, Faculty of Sciences, Pontificia Universidad Javeriana, Bogotá 110231, Colombia

2. Chemistry Department, Faculty of Sciences, Pontificia Universidad Javeriana, Bogotá 110231, Colombia

3. Faculty of Engineering and Basic Sciences, Insitituto Tecnológico del Putumayo, Mocoa 860001, Colombia

4. Faculty of Sciences, Universidad Nacional of Colombia, Bogotá 111321, Colombia

Abstract

The antifungal activity of palindromic peptide RWQWRWQWR and its derivatives was evaluated against clinical isolates of Candida albicans and C. auris. Also, Bidens pilosa ethanolic extracts of leaves and stem were evaluated. Furthermore, combinations of peptide, extract, and/or fluconazole (FLC) were evaluated. The cytotoxicity of peptides and extracts in erythrocytes and fibroblasts was determined. The original palindromic peptide, some derivative peptides, and the ethanolic extract of leaves of B. pilosa exhibited the highest activity in some of the strains evaluated. Synergy was obtained between the peptide and the FLC against C. auris 435. The combination of the extract and the original palindromic peptide against C. albicans SC5314, C. auris 435, and C. auris 537 decreased the minimal inhibitory concentrations (MICs) by a factor of between 4 and 16. These mixtures induced changes in cell morphology, such as deformations on the cell surface. The results suggest that the combination of RWQWRWQWR and B. pilosa extract is an alternative for enhancing antifungal activity and decreasing cytotoxicity and costs and should be considered to be a promising strategy for treating diseases caused by Candida spp.

Funder

COLCIENCIAS

Diseño de una formulación para el tratamiento de la candidiasis invasiva multidrogoresistente, basada en péptidos de LfcinB libres o nanoencapsulados

World Bank

Fondo de Ciencia, Tecnología e Innovación del Sistema General de Regalías, en el marco del Programa de Becas de Excelencia Doctoral del Bicentenario

Publisher

MDPI AG

Subject

Plant Science,Ecology, Evolution, Behavior and Systematics,Microbiology (medical)

Link

https://www.mdpi.com/2309-608X/9/8/817/pdf

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