Unprecedented Antimicrobial and Cytotoxic Polyketides from Cultures of Diaporthe africana sp. nov.
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Published:2023-07-24
Issue:7
Volume:9
Page:781
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ISSN:2309-608X
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Container-title:Journal of Fungi
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language:en
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Short-container-title:JoF
Author:
Matio Kemkuignou Blondelle12, Lambert Christopher13ORCID, Stadler Marc12ORCID, Kouam Fogue Simeon4ORCID, Marin-Felix Yasmina12ORCID
Affiliation:
1. Department of Microbial Drugs, Helmholtz Centre for Infection Research (HZI) and German Centre for Infection Research (DZIF), Partner Site Hannover/Braunschweig, Inhoffenstrasse 7, 38124 Braunschweig, Germany 2. Institute of Microbiology, Technische Universität Braunschweig, Spielmannstraße 7, 38106 Braunschweig, Germany 3. Department of Cell Biology, Helmholtz Centre for Infection Research (HZI), Inhoffenstrasse 7, 38124 Braunschweig, Germany 4. Department of Chemistry, Higher Teacher Training College, University of Yaoundé I, Yaoundé P.O. Box 47, Cameroon
Abstract
Four unprecedented polyketides named isoprenylisobenzofuran B (2), isoprenylisobenzofuran C1/C2 (3), diaporisoindole F1/F2 (4), and isochromophilonol A1/A2 (7) were isolated from ethyl acetate extracts of the newly described endophytic fungus Diaporthe africana. Additionally, the previously reported cyclic depsipeptide eucalactam B (1) was also identified, along with the known compounds diaporisoindole A/B (5), tenellone B (6) and beauvericin (8). The taxonomic identification of the fungus was accomplished using a polyphasic approach combining multi-gene phylogenetic analysis and microscopic morphological characters. The structures 1–8 were determined by a detailed analysis of their spectral data, namely high-resolution electrospray ionization mass spectrometry (HR-ESIMS), 1D/2D nuclear magnetic resonance (NMR) spectroscopy, as well as electronic circular dichroism (ECD) spectra. In addition, chemical methods such as Marfey’s analysis were also employed to determine the stereochemistry in compound 1. All the compounds obtained were evaluated for antimicrobial and in vitro cytotoxic properties. Compounds 3–8 were active against certain fungi and Gram-positive bacteria with MIC values of 8.3 to 66.6 µg/mL. In addition, 3–5 displayed cytotoxic effects (22.0 ≤ IC50 ≤ 59.2 µM) against KB3.1 and L929 cell lines, whereas compounds 6–8 inhibited the growth of seven mammalian cancer cell lines with IC50 ranging from 17.7 to 49.5 µM (6), 0.9 to 12.9 µM (7) and 1.9 to 4.3 µM (8).
Funder
personal PhD stipend from the German Academic Exchange Service Deutsche Forschungsgemeinschaft The World Academy of Sciences AvH Foundation Research Group Linkage AvH Research hub project CECANAPROF Life Science Foundation, Braunschweig, Germany
Subject
Plant Science,Ecology, Evolution, Behavior and Systematics,Microbiology (medical)
Reference84 articles.
1. Xu, T.C., Lu, Y.H., Wang, J.F., Song, Z.Q., Hou, Y.G., Liu, S.S., Liu, C.S., and Wu, S.H. (2021). Bioactive secondary metabolites of the genus Diaporthe and anamorph Phomopsis from terrestrial and marine habitats and endophytes: 2010–2019. Microorganisms, 9. 2. The genus Diaporthe: A rich source of diverse and bioactive metabolites;Chepkirui;Mycol. Prog.,2017 3. Catalano, A., Iacopetta, D., Ceramella, J., Scumaci, D., Giuzio, F., Saturnino, C., Aquaro, S., Rosano, C., and Sinicropi, M.S. (2022). Multidrug resistance (MDR): A widespread phenomenon in pharmacological therapies. Molecules, 27. 4. Hilário, S., and Gonçalves, M.F.M. (2022). Endophytic Diaporthe as promising leads for the development of biopesticides and biofertilizers for a sustainable agriculture. Microorganisms, 10. 5. Wei, W., Khan, B., Dai, Q., Lin, J., Kang, L., Rajput, N.A., Yan, W., and Liu, G. (2023). Potential of secondary metabolites of Diaporthe species associated with terrestrial and marine origins. J. Fungi, 9.
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