Proteomic Analysis Reveals the Effects of Different Dietary Protein Levels on Growth and Development of Jersey-Yak
Author:
Yang Guowu12, Dai Rongfeng1, Ma Xiaoming1, Huang Chun1, Ma Xiaoyong1, Li Xinyi12, La Yongfu1, Dingkao Renqing3, Renqing Ji4, Guo Xian1ORCID, Zhaxi Ta5, Liang Chunnian1
Affiliation:
1. Key Laboratory of Animal Genetics and Breeding on Tibetan Plateau, Ministry of Agriculture and Rural Affairs, Key Laboratory of Yak Breeding Engineering of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, China 2. College of Life Sciences and Engineering, Northwest Minzu University, Lanzhou 730106, China 3. Animal Husbandry Station, Gannan Tibetan Autonomous Prefecture, Hezuo 747099, China 4. Zogemanma Town Animal Husbandry and Veterinary Station, Hezuo 747003, China 5. Qilian County Animal Husbandry Veterinary Workstation, Haibei Prefecture, Qilian 810400, China
Abstract
Jersey-yak is a hybrid offspring of Jersey cattle and yak (Bos grunniens). Changing the feeding system of Jersey-yak can significantly improve its growth performance. In this study, tandem mass tag (TMT) proteomics technology was used to determine the differentially expressed proteins (DEPs) of the longissimus lumborum (LL) muscle of Jersey-yak fed different protein levels of diet. The results showed that compared with the traditional grazing feeding, the growth performance of Jersey-yaks was significantly improved by crude protein supplementation after grazing. A total of 3368 proteins were detected in these muscle samples, of which 3365 were quantified. A total of 434 DEPs were identified. Through analyses, it was found that some pathways related to muscle growth and development were significantly enriched, such as Rap1 signaling pathway, mTOR signaling pathway, and TGF-beta signaling pathway. A number of DEPs enriched in these pathways are related to muscle cell development, differentiation, and muscle development, including integrin subunit alpha 7 (ITGA7), myosin heavy chain 8 (MYH8), and collagen type XII alpha 1 chain (COL12A1). In conclusion, the results of this study provide insights into the proteomics of different feeding patterns of Jersey-yak, providing a stronger basis for further understanding the biological mechanism of hybrid varieties.
Funder
National Key Research Program Modern Beef Cattle and Yak Industry Technology System Gansu Provincial Basic Research Innovation Group Project Hezuo City Yak Germplasm Improvement and Quality Improvement Project
Subject
General Veterinary,Animal Science and Zoology
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