Comparative Genetic Characterization of Pathogenic Escherichia coli Isolated from Patients and Swine Suffering from Diarrhea in Korea
Author:
Do Kyung-Hyo1, Seo Kwangwon1, Jung Myunghwan2ORCID, Lee Woo-Kon2ORCID, Lee Wan-Kyu13
Affiliation:
1. College of Veterinary Medicine, Chungbuk National University, Cheongju 28644, Republic of Korea 2. Department of Microbiology, College of Medicine, Gyeongsang National University, Jinju 52727, Republic of Korea 3. GutBiomeTech, Co., Ltd., Cheongju 28644, Republic of Korea
Abstract
The aim of this study was to compare the virulence factors and antimicrobial resistance of the most common pathogenic Escherichia coli strains in swine and patients with diarrhea in Korea. We examined virulence genes and antimicrobial susceptibility in 85 and 61 E. coli strains isolated from swine and patients with diarrhea, respectively. The most prevalent pathogen in swine was enterotoxigenic E. coli (ETEC) (47.1%), followed by Shiga toxin-producing E. coli (STEC) (32.9%). Similarly, the majority of the patient isolates (50.8%) were proven to be STEC, the most common pathotype, followed by ETEC (23.0%). We found that swine isolates had significantly higher resistance than patient isolates, especially to fluoroquinolones (ciprofloxacin: 37.5% and 16.1%; norfloxacin: 29.7% and 16.1%, respectively). Additionally, sequence type (ST) 100 (swine: 21; patients: 4), ST 1 (swine: 21, patients: 2), ST 10 (swine: 8; patients: 6), ST 641 (swine: 3, patients: 2), and ST 88 (swine: 2, patients: 11) were detected in both swine and humans. In addition, we confirmed that isolates from swine and patients had similar virulence traits and were phylogenetically similar. According to these findings, swine and humans are susceptible to cross infection and the transfer of antimicrobial resistance.
Funder
Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries National Research Foundation of Korea
Subject
General Veterinary,Animal Science and Zoology
Reference55 articles.
1. Rhouma, M., Beaudry, F., Thériault, W., Bergeron, N., Laurent-Lewandowski, S., Fairbrother, J.M., and Letellier, A. (2005). Impacts of colistin sulfate on fecal Escherichia coli resistance and on growth. Safepork Posters, 361–365. 2. Antimicrobial resistance, adhesin and toxin genes of porcine pathogenic Escherichia coli following the ban on antibiotics as the growth promoters in feed;Do;Pak. Vet. J.,2021 3. Joseph, A., Cointe, A., Mariani Kurkdjian, P., Rafat, C., and Hertig, A. (2020). Shiga toxin-associated hemolytic uremic syndrome: A narrative review. Toxins, 12. 4. European Food Safety Authority, and European Centre for Disease Prevention and Control (2021). The European Union Summary Report on antimicrobial Resistance in zoonotic and indicator bacteria from humans, animals and food in 2018/2019. EFSA J., 19, e06490. 5. Animal and Plant Quarantine Agency (APQA) (2019). Antimicrobial Use and Antimicrobial Resistance Monitoring in Animals and Animal Products, Animal and Plant Quarantine Agency (APQA).
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