In Vivo Microbeam Radiation Therapy at a Conventional Small Animal Irradiator

Author:

Ahmed Mabroor123ORCID,Bicher Sandra12,Combs Stephanie Elisabeth12,Lindner Rainer2,Raulefs Susanne12,Schmid Thomas E.12,Spasova Suzana123,Stolz Jessica12,Wilkens Jan Jakob13ORCID,Winter Johanna1234ORCID,Bartzsch Stefan124ORCID

Affiliation:

1. Department of Radiation Oncology, School of Medicine and Klinikum rechts der Isar, Technical University of Munich, 81675 Munich, Germany

2. Helmholtz Zentrum München GmbH, German Research Center for Environmental Health, Institute of Radiation Medicine, 85764 Neuherberg, Germany

3. Department of Physics, School of Natural Sciences, Technical University of Munich, 85748 Garching, Germany

4. Heinz Maier-Leibnitz Zentrum (MLZ), 85748 Garching, Germany

Abstract

Microbeam radiation therapy (MRT) is a still pre-clinical form of spatially fractionated radiotherapy, which uses an array of micrometer-wide, planar beams of X-ray radiation. The dose modulation in MRT has proven effective in the treatment of tumors while being well tolerated by normal tissue. Research on understanding the underlying biological mechanisms mostly requires large third-generation synchrotrons. In this study, we aimed to develop a preclinical treatment environment that would allow MRT independent of synchrotrons. We built a compact microbeam setup for pre-clinical experiments within a small animal irradiator and present in vivo MRT application, including treatment planning, dosimetry, and animal positioning. The brain of an immobilized mouse was treated with MRT, excised, and immunohistochemically stained against γH2AX for DNA double-strand breaks. We developed a comprehensive treatment planning system by adjusting an existing dose calculation algorithm to our setup and attaching it to the open-source software 3D-Slicer. Predicted doses in treatment planning agreed within 10% with film dosimetry readings. We demonstrated the feasibility of MRT exposures in vivo at a compact source and showed that the microbeam pattern is observable in histological sections of a mouse brain. The platform developed in this study will be used for pre-clinical research of MRT.

Publisher

MDPI AG

Subject

Cancer Research,Oncology

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