Impact of Prior Pulsed Electric Field and Chitooligosaccharide Treatment on Trypsin Activity and Quality Changes in Whole and Beheaded Harpiosquillid Mantis Shrimp during Storage in Iced Water
Author:
Chanchi Prashanthkumar Mallikarjun1, Temdee Wattana1, Mittal Ajay1, Suyapoh Watcharapol2, Sornying Peerapon2, Palamae Suriya1ORCID, Bautong Jirayu1, Zhang Bin3, Hong Hui4ORCID, Benjakul Soottawat1ORCID
Affiliation:
1. International Center of Excellence in Seafood Science and Innovation, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai 90110, Thailand 2. Veterinary Pathology Unit, Department of Veterinary Science, Faculty of Veterinary Science, Prince of Songkla University, Hat Yai 90110, Thailand 3. Key Laboratory of Health Risk Factors for Seafood of Zhejiang Province, College of Food Science and Pharmacy, Zhejiang Ocean University, Zhoushan 316022, China 4. Beijing Laboratory for Food Quality and Safety, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China
Abstract
Harpiosquillid mantis shrimp (Harpiosquilla raphidea) (HMS) without and with beheading pretreated with pulsed electric field (PEF) (15 kV/cm, 800 pulses, 5 min) were soaked in chitooligosaccharide (COS) solution at varying concentrations (0, 1 and 2%, w/v) for 20 min and stored for 3 days in iced water. Changes in the trypsin activity, color, texture, protein pattern, TCA soluble peptide content, histological images, protein secondary structure and microbial load were monitored during the storage. The beheaded HMS pretreated with PEF and soaked in 2% COS solution showed the maximum efficacy in inhibiting trypsin activity and proteolysis, thus retaining muscle proteins, especially myosin heavy chain, actin and troponin T as well as shear force up to day 3. Pronounced muscle destruction in the whole HMS was displayed by a decreased mean grey index and fiber gapping. Such changes were lowered by the beheading and PEF/2% COS treatment (2% COS-BH). Nevertheless, no marked change in the secondary structure including α-helix, β-sheets, β-turns and random coil were observed among any of the samples. The microbiological analysis revealed that the total viable count (TVC) was below 6 log CFU/g till day 2 in all samples. Nonetheless, the 2% COS-BH sample had the lowest psychrophilic bacterial count and Enterobacteriaceae count at day 3, compared to the others. Thus, the combination of the prior PEF and 2% COS treatment of beheaded HMS could effectively inhibit proteases, retard the microbial growth and maintain the quality of HMS stored in iced water.
Funder
Reinventing University Program by Office of the Permanent Secretary, Ministry of Higher Education, Science, Research and Innovation, Thailand Prince of Songkla University
Subject
Plant Science,Health Professions (miscellaneous),Health (social science),Microbiology,Food Science
Reference29 articles.
1. Marine biodiversity of the South China Sea a Checklist of Stomatopod Crustacea;Kasim;Raffles Bull. Zool.,2000 2. Rapid Quality Deterioration of Harpiosquillid Mantis Shrimp (Harpiosquilla raphidea) during Iced Storage;Temdee;J. Food Sci. Technol.,2022 3. Effects of Proteases on Textural Softening and Changes in Physical Property of Harpiosquillid Mantis Shrimp (Harpiosquilla raphidea) during the Chilled Storage;Temdee;Int. J. Food Sci. Technol.,2023 4. Effect of Heat Treatment at Different Stages of Rigor on the Quality of Pacific White Shrimp (Litopenaeus vannamei);Bhat;J. Aquat. Food Prod. Technol.,2017 5. Zhang, S., Meenu, M., Hu, L., Ren, J., Ramaswamy, H.S., and Yu, Y. (2022). Recent Progress in the Synergistic Bactericidal Effect of High Pressure and Temperature Processing in Fuits and Vegetables and Related Kinetics. Foods, 11.
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