Genetic Characterization of African Swine Fever Italian Clusters in the 2022–2023 Epidemic Wave by a Multi-Gene Approach-Reference-Cited by-同舟云学术

Genetic Characterization of African Swine Fever Italian Clusters in the 2022–2023 Epidemic Wave by a Multi-Gene Approach

Published:2024-07-24 Issue:8 Volume:16 Page:1185
ISSN:1999-4915
Container-title:Viruses
language:en
Short-container-title:Viruses

Author:

Giammarioli Monica¹^ORCID,Torresi Claudia¹^ORCID,Biccheri Roberta¹^ORCID,Cammà Cesare²^ORCID,Marcacci Maurilia²^ORCID,Dondo Alessandro³,Razzuoli Elisabetta³^ORCID,Fusco Giovanna⁴^ORCID,Casalinuovo Francesco⁴,Scicluna Maria Teresa⁵,Dei Giudici Silvia⁶^ORCID,Martin Ana Maria Moreno⁷^ORCID,Rossi Elisabetta¹,Casciari Cristina¹,Pela Michela¹,Iscaro Carmen¹^ORCID,Gallardo Carmina⁸^ORCID,Marocco Gaia¹,Orrico Mario⁹,Feliziani Francesco¹^ORCID

Affiliation:

1. National Reference Laboratory (NRL) for Swine Fever, Istituto Zooprofilattico Sperimentale dell’Umbria e delle Marche “Togo Rosati”, 06126 Perugia, Italy

2. National Reference Center for Whole Genome Sequencing of Microbial Pathogens, Database and Bioinformatic Analysis, Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise “G. Caporale”, 64100 Teramo, Italy

3. Istituto Zooprofilattico Sperimentale Piemonte, Liguria e Valle d’Aosta “I. Altara”, 10154 Turin, Italy

4. Istituto Zooprofilattico Sperimentale del Mezzogiorno, Portici, 80055 Napoli, Italy

5. Istituto Zooprofilattico Sperimentale del Lazio e della Toscana “M. Aleandri”, 00178 Rome, Italy

6. Istituto Zooprofilattico Sperimentale della Sardegna “G. Pegreffi”, 07100 Sassari, Italy

7. Istituto Zooprofilattico Sperimentale della Lombardia e della Emilia Romagna “Bruno Ubertini”, 25124 Brescia, Italy

8. European Union Reference Laboratory for African Swine Fever (EURL), Centro de Investigación en Sanidad Animal (CISA), Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Consejo Superior de Investigaciones Científicas (CSIC), Valdeolmos, 28130 Madrid, Spain

9. Veterinary Practitioner, 87040 Cosenza, Italy

Abstract

The first report of African swine fever virus (ASFV) genotype II in Italy in 2022 marked the beginning of a significant invasion in at least eight Italian regions with different infection clusters. In this study, we used the multi-gene approach to investigate the epidemiological associations between ASFV strains causing cases and outbreaks in wild boar and pigs in Italy from January 2022 to the end of 2023. Our results confirm that all the tested ASFV-positive Italian samples belonged to genotype II and show high homology with genotype II ASFV sequences previously collected in Eurasian countries. Molecular characterization revealed the presence of four genetic groups in Italy. The majority of African swine fever (ASF) samples analyzed in the current study (72%) belonged to genetic group 3, which was the most representative in Europe. The results also provide evidence of the prevalence of genetic group 19 (15.9%). In addition, we identified new putative genetic groups, genetic group 25 (9.1%) and genetic group 26 (3.0%), which have never been described before. This is the first detailed report on the molecular characterization of more than 130 ASFV strains circulating in Italy.

Funder

Istituto Zooprofilattico Sperimentale dell’ Umbria e delle Marche “Togo Rosati”

Publisher

MDPI AG

Link

https://www.mdpi.com/1999-4915/16/8/1185/pdf

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