Conversion of Human Umbilical Cord Mesenchymal Stem Cells in Wharton's Jelly to Dopaminergic Neurons In Vitro: Potential Therapeutic Application for Parkinsonism-Reference-Cited by-同舟云学术

Conversion of Human Umbilical Cord Mesenchymal Stem Cells in Wharton's Jelly to Dopaminergic Neurons In Vitro: Potential Therapeutic Application for Parkinsonism

Published:2005-08-11 Issue:1 Volume:24 Page:115-124
ISSN:1066-5099
Container-title:Stem Cells
language:en
Short-container-title:

Author:

Fu Yu-Show¹,Cheng Yun-Chih²,Lin Maan-Yuh Anya³⁴,Cheng Henrich⁵⁶⁷,Chu Pei-Ming²,Chou Shih-Chich²,Shih Yang-Hsin⁸⁹,Ko Miau-Hwa¹⁰,Sung Min-Shan¹¹

Affiliation:

1. Department of Anatomy, National Yang-Ming University, Taipei, Taiwan

2. Institute of Anatomy and Cell Biology, National Yang-Ming University, Taipei, Taiwan

3. Institute of Physiology, School of Medicine, National Yang-Ming University, Taipei, Taiwan

4. Department of Medicine Research and Education, Taipei Veterans General Hospital, Taipei, Taiwan

5. Center for Neural Regeneration, Neurological Institute, Taipei Veterans General Hospital, Taipei, Taiwan

6. Neural Regeneration Laboratory, Neurological Institute, Taipei Veterans General Hospital, Taipei, Taiwan

7. Department of Pharmacology, School of Medicine, National Yang-Ming University, Taiwan

8. Department of Neurosurgery, Neurological Institute, Taipei Veterans General Hospital, Taipei, Taiwan

9. School of Medicine, Taipei Medical University, Taipei, Taiwan

10. Department of Anatomy, China Medical University, Taichung, Taiwan

11. Institute of Medical Science, China Medical University, Taichung, Taiwan

Abstract

Abstract Human mesenchymal stem cells isolated from Wharton's jelly of the umbilical cord were induced to transform into dopaminergic neurons in vitro through stepwise culturing in neuron-conditioned medium, sonic hedgehog, and FGF8. The success rate was 12.7%, as characterized by positive staining for tyrosine hydroxylase (TH), the rate-limiting catecholaminergic synthesizing enzyme, and dopamine being released into the culture medium. Transplantation of such cells into the striatum of rats previously made Parkinsonian by unilateral striatal lesioning with the dopaminergic neurotoxin 6-hydroxydopamine partially corrected the lesion-induced amphetamine-evoked rotation. Viability of the transplanted cells at least 4 months after transplantation was identified by positive TH staining and migration of 1.4 mm both rostrally and caudally. These results suggest that human umbilical mesenchymal stem cells have the potential for treatment of Parkinson's disease.

Publisher

Oxford University Press (OUP)

Subject

Cell Biology,Developmental Biology,Molecular Medicine

Link

https://onlinelibrary.wiley.com/doi/pdf/10.1634/stemcells.2005-0053

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