Human Dental Pulp Stem Cells Improve Left Ventricular Function, Induce Angiogenesis, and Reduce Infarct Size in Rats with Acute Myocardial Infarction

Author:

Gandia Carolina1,Armiñan Ana1,García-Verdugo Jose Manuel12,Lledó Elisa1,Ruiz Amparo2,Miñana M Dolores3,Sanchez-Torrijos Jorge3,Payá Rafael3,Mirabet Vicente4,Carbonell-Uberos Francisco4,Llop Mauro1,Montero Jose Anastasio15,Sepúlveda Pilar13

Affiliation:

1. Unidad de Cardiorregeneración, Centro de Investigación Príncipe Felipe, Valencia, Spain

2. Universidad de Valencia, Valencia, Spain

3. Fundación Hospital General Universitario, Valencia, Spain

4. Centro de Transfusion de la Comunidad Valenciana, Valencia, Spain

5. Servicio de Cirugía Cardiaca, Hospital Universitario La Fe, Valencia, Spain

Abstract

Abstract Human dental pulp contains precursor cells termed dental pulp stem cells (DPSC) that show self-renewal and multilineage differentiation and also secrete multiple proangiogenic and antiapoptotic factors. To examine whether these cells could have therapeutic potential in the repair of myocardial infarction (MI), DPSC were infected with a retrovirus encoding the green fluorescent protein (GFP) and expanded ex vivo. Seven days after induction of myocardial infarction by coronary artery ligation, 1.5 × 106 GFP-DPSC were injected intramyocardially in nude rats. At 4 weeks, cell-treated animals showed an improvement in cardiac function, observed by percentage changes in anterior wall thickening left ventricular fractional area change, in parallel with a reduction in infarct size. No histologic evidence was seen of GFP+ endothelial cells, smooth muscle cells, or cardiac muscle cells within the infarct. However, angiogenesis was increased relative to control-treated animals. Taken together, these data suggest that DPSC could provide a novel alternative cell population for cardiac repair, at least in the setting of acute MI. Disclosure of potential conflicts of interest is found at the end of this article.

Publisher

Oxford University Press (OUP)

Subject

Cell Biology,Developmental Biology,Molecular Medicine

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