Ginsenoside-Rb1 from Panax ginseng C.A. Meyer Activates Estrogen Receptor-α and -β, Independent of Ligand Binding

Author:

Cho JungYoon1,Park Wankyu1,Lee SeungKi2,Ahn Woongshick3,Lee YoungJoo1

Affiliation:

1. College of Life Science (JY.C., W.P., YJ.L.), Institute of Biotechnology, Department of Bioscience and Biotechnology, Sejong University, Seoul 143-747, Korea

2. College of Pharmacy (SK.L.), Seoul National University, Seoul 151-742, Korea

3. Department of Obstetrics and Gynecology (W.A.), Catholic Research Institutes of Medical Science, College of Medicine, The Catholic University of Korea, Seoul 137-701, Korea

Abstract

Abstract We studied the estrogenic activity of a component of Panax ginseng, ginsenoside-Rb1. The activity of ginsenoside-Rb1 was characterized in a transient transfection system, using estrogen receptor isoforms and estrogen-responsive luciferase plasmids, in COS monkey kidney cells. Ginsenoside-Rb1 activated both α and β estrogen receptors in a dose-dependent manner with maximal activity observed at 100 μm, the highest concentration examined. Activation was inhibited by the estrogen receptor antagonist ICI 182,780, indicating that the effects were mediated through the estrogen receptor. Treatment with 17β-estradiol or ginsenoside-Rb1 increased expression of the progesterone receptor, pS2, and estrogen receptor in MCF-7 cells and of AP-1-driven luciferase genes in COS cells. Although these data suggest that it is functionally very similar to 17β-estradiol, ginsenoside-Rb1 failed to displace specific binding of [3H]17β-estradiol from estrogen receptors in MCF-7 whole-cell ligand binding assays. Our results indicate that the estrogen-like activity of ginsenoside-Rb1 is independent of direct estrogen receptor association.

Publisher

The Endocrine Society

Subject

Biochemistry (medical),Clinical Biochemistry,Endocrinology,Biochemistry,Endocrinology, Diabetes and Metabolism

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