Affiliation:
1. Federal Scientific Center for Medical and Preventive Health Risk Management Technologies
Abstract
Introduction. Phenol and its derivatives are widely distributed in the environment. To assess the risk of the negative impact of phenols on human health, data on their content in biological media are needed.
Purpose of research. Development of a sensitive and selective method for the determination of phenol and catechol in the whole blood by HPLC.
Materials and methods. The studies were carried out on a Shimadzu liquid chromatograph with an RF-20A fluorimetric detector. The efficiency of extraction of analytes from the matrix was checked by methods of liquid and solid phase extraction, QuEChERS. The metrological parameters of the measurement technique were experimentally established. Approbation of the method was carried out during the analysis of whole blood in children living in territories with various technogenic impacts.
Results. The developed method makes it possible to determine phenol and catechol in whole blood at the level of 0.005–0.5 mg/dm3 with an error of ≤33%. The degree of extraction of phenol from whole blood by the QuEChERS method is 100%, pyrocatechol – 75%. A significantly higher (p ≤ 0.05) average group content of phenol and pyrocatechol was established in the whole blood of children living in an ecologically loaded area compared to a conditionally clean area by 2.1 times.
Limitations. The study of the content of phenol and pyrocatechol in the whole blood in the child population is limited by the number of territories and examined children. To establish the background content of phenol and catechol in the whole blood of the child population at the population level under conditions of environmental stress and outside the zone of anthropogenic influence, it is necessary to conduct more extensive studies in various territories covering a larger number of examined children.
Conclusion. The developed method can be used in hygienic research to assess the risk of phenols exposure to the health of the child population living in areas with various anthropogenic pressure.
Publisher
Federal Scientific Center for Hygiene F.F.Erisman
Subject
Health, Toxicology and Mutagenesis,Public Health, Environmental and Occupational Health,Pollution,General Medicine
Reference34 articles.
1. Nekrasova L.P., Malysheva A.G., Abramov E.G. Transformation of phenol and diatomic phenols in surface water under the impact of natural physical and chemical factors. Gigiena i Sanitaria (Hygiene and Sanitation, Russian journal). 2019; 98(11): 1206–11. https://doi.org/10.18821/0016-9900-2019-98-11-1206-1211 https://elibrary.ru/qbyray (in Russian)
2. Makhmudova K.K., Masaev Yu.A., Anisimova A.V. Industrial processing of stone coal and its influence on ecology of the environment. In: Modern Trends and Innovations in Science and Production. Materials of the IX International Scientific and Practical Conference [Sovremennye tendentsii i innovatsii v nauke i proizvodstve. Materialy IX mezhdunarodnoy nauchno-prakticheskoy konferentsii]. Kemerovo; 2020. https://elibrary.ru/ymnnfk (in Russian)
3. Timofeeva E.E. Emissions of pollutants from coke production into the atmospheric air. In: Integration Processes in Science in the XXI Century. Materials of the International (Correspondence) Scientific and Practical Conference [Integratsionnye protsessy v nauke v XXI veke. Materialy Mezhdunarodnoy (zaochnoy) nauchno-prakticheskoy konferentsii]. Neftekamsk; 2018: 17–21. https://elibrary.ru/lwuful (in Russian)
4. Baktybaeva Z.B., Suleymanov R.A., Valeev T.K., Rakhmatullin N.R., Stepanov E.G., Davletnurov N.Kh. Environmental and hygienic assessment of ambient air pollution in oil-producing and oil-refining areas of the Republic of Bashkortostan and population health status. Zdorov’e naseleniya i sreda obitaniya – ZNiSO. 2020; (2): 26–32. https://doi.org/10.35627/2219-5238/2020-323-2-26-32 https://elibrary.ru/xdsvzr (in Russian)
5. Shmukler E.G. The chemical composition of tobacco smoke as a factor in the vital activity of the human body. Khimiya. 2009; (2): 29. (in Russian)