Affiliation:
1. Department of Cellular and Molecular Biology Fujita Health University School of Medical Sciences Toyoake Aichi Japan
2. Department of Pediatrics Kariya Toyota General Hospital Kariya Aichi Japan
3. Department of Pediatrics Fujita Health University School of Medicine Toyoake Aichi Japan
4. Department of Clinical Science for Biological Monitoring Fujita Health University School of Medical Sciences Toyoake Aichi Japan
Abstract
AbstractIn the era of universal varicella vaccination, diagnosis of varicella is challenging, especially for breakthrough cases. We sought to clarify the reliability of direct varicella‐zoster virus (VZV) loop‐mediated isothermal amplification (LAMP) and DermaQuick® VZV using the immunochromatography technique as rapid diagnostic tests for varicella. In addition, the usefulness of saliva as a sample type for direct LAMP was investigated. Among the 46 enrolled patients with suspected VZV infection, 31 patients (67.3%) were positive for the nucleic acid test based on real‐time PCR from skin swab samples. Direct LAMP of skin swabs was positive in 29 (63.0%) of 46 patients. DermaQuick® VZV was positive in 25 (54.3%) of 46 patients. VZV DNA was detected in only 48.4% of oral swabs with the direct LAMP method. With real‐time polymerase chain reaction (PCR) as the standard for diagnosing varicella, the sensitivity and specificity of DermaQuick® VZV were 80.7% and 100%, respectively. The sensitivity and specificity of direct LAMP from skin swabs were 93.6% and 100%, respectively. The sensitivity and specificity of real‐time PCR for DNA extracted from oral swabs were 74.2% and 93.3%, respectively. Thus, oral swab samples are not suitable for breakthrough varicella diagnosis. Although DermaQuick® VZV is considered the most convenient point‐of‐care test for varicella, its sensitivity and specificity were lower than those of direct VZV LAMP.
Funder
Japan Society for the Promotion of Science
Fujita Health University
Subject
Infectious Diseases,Virology
Reference36 articles.
1. LIVE VACCINE USED TO PREVENT THE SPREAD OF VARICELLA IN CHILDREN IN HOSPITAL
2. Varicella vaccination in Japan: necessity of implementing a routine vaccination program
3. Prevention of herpes zoster: recommendations of the Advisory Committee on Immunization Practices (ACIP);Harpaz R;MMWR Recomm Rep,1996
4. Recommendations for the use of live attenuated varicella vaccine. American Academy of Pediatrics Committee on Infectious Diseases;Committee on Infectious Diseases;Pediatrics,1995
5. Varicella Disease After Introduction of Varicella Vaccine in the United States, 1995-2000
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