Utilizing fab fragment‐conjugated surface plasmon resonance‐based biosensor for detection of SalmonellaEnteritidis

Author:

Eser Esma1ORCID,Ekiz Okan Öner23ORCID,Ekiz H. İbrahim4ORCID

Affiliation:

1. Department of Food Engineering Canakkale Onsekiz Mart University Çanakkale Turkey

2. Department of Material Science and Engineering OSTİM Teknical University Ankara Turkey

3. Nanodev Scientific Bilkent Cyberpark Ankara Turkey

4. Department of Food Engineering Mersin University Mersin Turkey

Abstract

AbstractAlthough antibodies, a key element of biorecognition, are frequently used as biosensor probes, the use of these large molecules can lead to adverse effects. Fab fragments can be reduced to allow proper antigen‐binding orientation via thiol groups containing Fab sites that can directly penetrate Au sites chemically. In this study, the ability of the surface plasmon resonance (SPR) sensor to detect Salmonella was studied. Tris(2‐carboxyethyl)phosphine was used as a reducing agent to obtain half antibody fragments. Sensor surface was immobilized with antibody, and bacteria suspensions were injected from low to high concentrations. Response units were changed by binding first reduced antibody fragments, then bacteria. The biosensor was able to determine the bacterial concentrations between 103 and 108 CFU/mL. Based on these results, the half antibody fragmentation method can be generalized for faster, label‐free, sensitive, and selective detection of other bacteria species.

Funder

Türkiye Bilimsel ve Teknolojik Araştırma Kurumu

Publisher

Wiley

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