Cellular Assays and Their Application in Drug Discovery

Abstract

AbstractOriginally published in: Molecular Biology in Medicinal Chemistry. Edited by Theodor Dingermann, Dieter Steinhilber and Gerd Folkers. Copyright © 2004 Wiley‐VCH Verlag GmbH & Co. KGaA Weinheim. Print ISBN: 3‐527‐30431‐8The sections in this article areIntroductionPositioning Cellular AssaysImpact on Drug DiscoveryClassification of Cellular AssaysProgress in Tools and Technologies for Cellular Compound ProfilingMembrane Proteins and Fast Cellular ResponsesReceptorsFLIPR Technology for Detection of Intracellular Calcium ReleaseCompetitive Immunoassay for Detection of Intracellular cAMPEnzyme Fragment Complementation (EFC) TechnologyMembrane Transport ProteinsIon ChannelsMDR ProteinsGene and Protein Expression Profiling in High‐throughput FormatsReporter Gene Assays in Lead FindingReporter Gene Assays in Lead OptimizationSpatio‐temporal Assays and Subpopulation AnalysisPhosphorylation Stage‐specific AntibodiesTarget‐protein‐specific AntibodiesProtein–GFP FusionsFluorescence Resonance Energy Transfer (FRET)GPCR Activation using Bioluminescence Resonance Energy Transfer (BRET)Protein Fragment Complementation Assays (PCA)Phenotypic AssaysProliferation/Respiration/ToxicityApoptosisDifferentiationMonitoring Cell MetabolismOther Phenotypic AssaysAcknowledgments