Caged Luciferase Inhibitor‐Based Bioluminescence Switching Strategy Enables Efficient Detection of Serum APN Activity and the Identification of Its Roles in Metastasis of Non‐Small Cell Lung Cancer**

Author:

Li Yunzhi1ORCID,Wu Jiaxin1,Jin Chaoying1,Zhang Yiqiu1,Wang Jiyu2,Wang Xuecen3ORCID,Li Huixia2,Zhang Xiaoyue3,Liu Tingyu4,Zhou Deyuan2,Kuang Yukun2ORCID,Wu Weijian1,Wang Youqiao1,Ke Zunfu5ORCID,Bu Xianzhang16ORCID,Yue Xin34ORCID

Affiliation:

1. School of Pharmaceutical Sciences Sun Yat-sen University Guangzhou 510006 China

2. Division of Pulmonary and Critical Care Medicine The First Affiliated Hospital of Sun Yat-sen University Institute of Respiratory Diseases of Sun Yat-sen University Guangzhou 510080 China

3. Department of Radiation Oncology The First Affiliated Hospital Sun Yat-sen University Guangzhou 510080 China

4. State Key Laboratory of Oncology in South China Collaborative Innovation Center for Cancer Medicine Sun Yat-sen University Cancer Center Guangzhou 510060 China

5. Molecular Diagnosis and Gene Test Center The First Affiliated Hospital of Sun Yat-sen University Guangzhou 510080 China

6. State Key Laboratory of Chemical Oncogenomics Guangdong Provincial Key Laboratory of Chemical Genomics Peking University Shenzhen Graduate School Shenzhen 518055 China

Abstract

AbstractBioluminogenic probes emerged as powerful tools for imaging and analysis of various bioanalyses, but traditional approaches would be limited to the low sensitivity during determine the low activity of protease in clinical specimens. Herein, we proposed a caged luciferase inhibitor‐based bioluminescence‐switching strategy (CLIBS) by using a cleavable luciferase inhibitor to modulate the activity of luciferase reporter to amplify the detective signals, which led to the enhancement of detection sensitivity, and enabled the determination of circulating Aminopeptidase N (APN) activity in thousands of times diluted serum. By applying the CLIBS to serum samples in non–small cell lung cancer (NSCLC) patients from two clinical cohorts, we revealed that, for the first time, higher circulating APN activities but not its concentration, were associated with more NSCLC metastasis or higher metastasis stages by subsequent clinical analysis, and can serve as an independent factor for forecasting NSCLC patients’ risk of metastasis.

Funder

National Natural Science Foundation of China

Basic and Applied Basic Research Foundation of Guangdong Province

Publisher

Wiley

Subject

General Chemistry,Catalysis,Organic Chemistry

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3