Affiliation:
1. Department of Chemistry National Institute of Technology Rourkela 769008, Odisha India
2. Institut für Anorganische und Analytische Chemie Friedrich-Schiller-Universität Jena Humboldtstr. 8 07743 Jena Germany
Abstract
AbstractThe reaction of the Ru(PPh3)3Cl2 with HL1−3−OH (−OH stands for the oxime hydroxyl group; HL1−OH=diacetylmonoxime‐S‐benzyldithiocarbazonate; HL2−OH=diacetylmonoxime‐S‐(4‐methyl)benzyldithiocarbazonate; and HL3−OH=diacetylmonoxime‐S‐(4‐chloro)benzyl‐dithiocarbazonate) gives three new ruthenium complexes [RuII(L1−3−H)(PPh3)2Cl] (1–3) (−H stands for imine hydrogen) coordinated with dithiocarbazate imine as the final products. All ruthenium(II) complexes (1–3) have been characterized by elemental (CHNS) analyses, IR, UV‐vis, NMR (1H, 13C, and 31P) spectroscopy, HR‐ESI‐MS spectrometry and also, the structure of 1–2 was further confirmed by single crystal X‐ray crystallography. The solution/aqueous stability, hydrophobicity, DNA interactions, and cell viability studies of 1–3 against HeLa, HT‐29, and NIH‐3T3 cell lines were performed. Cell viability results suggested 3 being the most cytotoxic of the series with IC50 6.9±0.2 μM against HeLa cells. Further, an apoptotic mechanism of cell death was confirmed by cell cycle analysis and Annexin V‐FITC/PI double staining techniques. In this regard, the live cell confocal microscopy results revealed that compounds primarily target the mitochondria against HeLa, and HT‐29 cell lines. Moreover, these ruthenium complexes elevate the ROS level by inducing mitochondria targeting apoptotic cell death.
Funder
Council of Scientific and Industrial Research, India
Subject
General Chemistry,Catalysis,Organic Chemistry
Cited by
11 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献