Affiliation:
1. Institute for Organic Chemistry and BMWZ Leibniz Universität Hannover Schneiderberg 38 30167 Hannover Germany
Abstract
AbstractThree central steps during the biosynthesis of cytochalasan precursors, including reductive release, Knoevenagel cyclisation and Diels Alder cyclisation are not yet understood at a detailed molecular level. In this work we investigated the reductive release step catalysed by a hybrid polyketide synthase non‐ribosomal peptide synthetase (PKS‐NRPS) from the pyrichalasin H pathway. Synthetic thiolesters were used as substrate mimics for in vitro studies with the isolated reduction (R) and holo‐thiolation (T) domains of the PKS‐NRPS hybrid PyiS. These assays demonstrate that the PyiS R‐domain mainly catalyses an NADPH‐dependent reductive release of an aldehyde intermediate that quickly undergoes spontaneous Knoevenagel cyclisation. The R‐domain can only process substrates that are covalently bound to the phosphopantetheine thiol of the upstream T‐domain, but it shows little selectivity for the polyketide.
Funder
China Scholarship Council
Deutsche Forschungsgemeinschaft
Subject
General Chemistry,Catalysis,Organic Chemistry