Affiliation:
1. School of Chinese Materia Medica Beijing University of Chinese Medicine Beijing China
2. School of Life Sciences Beijing University of Chinese Medicine Beijing China
Abstract
AbstractBACKGROUNDNonalcoholic fatty liver disease (NAFLD) is the most common cause of liver cirrhosis and cancer. Lonicerae flos polysaccharides (LPs) have been shown to be effective in treating metabolic diseases; however, the therapeutic effects and underlying molecular mechanisms of LPs in NAFLD remain unclear.PURPOSEThe objective of this study was to investigate the morphological characterization of Lonicerae flos polysaccharides (LPs) and the mechanism of LPs in relieving NAFLD.METHODSThe morphology of LPs was observed using atomic force microscopy (AFM), X‐ray diffraction (XRD), thermal weight (TG), and thermal weight derivative (DTG); NAFLD mice were treated with LPs at the same time as they were induced with a Western diet, and then the indexes related to glycolipid metabolism, fibrosis, inflammation, and autophagy in the serum and liver of the mice were detected.RESULTSThe atomic force microscope analysis results indicated that the LPs displayed sugar‐chain aggregates, exhibited an amorphous structure, and were relatively stable in thermal cracking at 150 °C. It was also found that LPs exerted therapeutic effects in NAFLD. The LPs prevented high‐fat and ‐cholesterol diet‐induced NAFLD progression by regulating glucose metabolism dysregulation, insulin resistance, lipid accumulation, inflammation, fibrosis, and autophagy. Adenosine 5′‐monophosphate (AMP)‐activated protein kinase (AMPK) inhibitor compound C abrogated LP‐induced hepatoprotection in mice with NAFLD. The LPs further treated NAFLD by reshaping the structure of the gut microbiota, in which Desulfovibrio bacteria plays a key roles.CONCLUSIONLonicerae flos polysaccharides exert protective effects against NAFLD in mice by improving the structure of the intestinal flora and activating the AMPK signaling pathway. © 2023 Society of Chemical Industry.
Funder
National Natural Science Foundation of China
Subject
Nutrition and Dietetics,Agronomy and Crop Science,Food Science,Biotechnology
Cited by
1 articles.
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