Affiliation:
1. State Key Laboratory of Bioorganic and Natural Products Chemistry Shanghai Institute of Organic Chemistry Chinese Academy of Sciences Shanghai P. R. China
2. School of Physical Science and Technology Shanghai Tech University Shanghai P. R. China
3. School of Chemical Engineering Xi'an Key Laboratory of Food Safety Testing and Risk Assessment Xi'an University Xi'an P. R. China
Abstract
A method for the preparation of poly(N‐vinylpyrrolidone‐co‐pentaerythritol triacrylate copolymerization)‐based monolithic capillary column was reported for the separation of polar small molecular weight compounds with nano‐liquid chromatography in hydrophilic interaction chromatography mode. The monolithic columns were prepared by in situ copolymerization of N‐vinylpyrrolidone and a cross‐linker pentaerythritol triacrylate in a binary porogenic agent consisting of methanol and water. The composition of the polymerization solution was systematically optimized in terms of column permeability, theoretical plate number, asymmetric factor, and retention factor. A typical hydrophilic chromatography retention mechanism was observed with a mobile phase composed of a high content of organic solvent. The preparation method is simple and robust, the precursor N‐vinylpyrrolidone is chemically stable, cheap, and easily available. The N‐vinylpyrrolidone‐based hydrophilic interaction chromatography stationary phase displays satisfactory separation selectivity for a range of polar test analytes, including benzoic acid derivatives, nucleosides, and phenols.
Subject
Filtration and Separation,Analytical Chemistry