Exome sequencing identifies ADGRG4 G‐protein‐coupled receptors gene as a novel cancer biomarker in ovarian cancer patients from North India

Author:

Sharma Minerva1,Verma Sonali2,Angurana Shabab Lalit3,Tufail Ziya1,Bhagat Vanshika1,Nagyal Sonia4,Jamwal Rajeshwer Singh1,Sharma Bhawani1,Shah Ruchi5,Bhat Audesh6,Chander Gresh2,Kumar Rakesh12ORCID

Affiliation:

1. School of Biotechnology Shri Mata Vaishno Devi University Katra India

2. Indian Council of Medical Research‐Centre for Advance Research Shri Mata Vaishno Devi University Katra India

3. Department of Radiotherapy and Oncology AIIMS Vijaypur India

4. Department of Histopathology, Shri Mata Vaishno Devi Narayana Multispeciality Clinic Shri Mata Vaishno Devi Narayana Superspeciality Hospital Katra India

5. School of Biotechnology Shri Mata Vaishno Devi University, Katra, India Jammu & Kashmir India

6. Centre for Molecular Biology Central University of Jammu Jammu & Kashmir India

Abstract

AbstractAdhesion G protein‐coupled receptor G4 (ADGRG4) is a G protein‐coupled receptor (GPCR) that belongs to the adhesion family. Participation of ADGRG4 in cell adhesion and migration, signaling pathway activation, influence on angiogenesis, and modulation of immune responses are some of the possible ways through which it may contribute to oncogenesis. Conducting extensive omics studies poses budgetary challenges to small labs in peripheral areas, primarily due to restricted research funding and resource limitations. Here we propose a low‐budget model for biomarker screening. A total of 11 ovarian cancer samples were sent for exome sequencing. Among various genes, ADGRG4 variants were present in all 11 samples and thus were chosen as a potential biomarker in the present population. However, the precise role of ADGRG4 in cancer is not fully understood. The present study aims to look at the association between the ADGRG4 gene variants and their risk of ovarian cancer in the North Indian region of Jammu and Kashmir, India. Overall, 235 individuals (115 cases and 120 healthy controls) were genotyped for the selected biomarker using Sanger sequencing. Logistic regression was used to assess the relationship between the variant and ovarian cancer. A statistically significant association was identified between the ADGRG4 variant rs5930932 polymorphism and the incidence of ovarian cancer among the study population. When corrected for age and BMI, the dominating OR of variant rs5930932 was 1.035 (1.003−1.069) under HWE patients (0.95) and controls (0.18), with a p‐value of (0.03). According to the findings of the current investigation, the ADGRG4 gene variant rs5930932 increases the chance of developing ovarian cancer in the studied population.

Publisher

Wiley

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3