Affiliation:
1. School of Chinese Materia Medica Tianjin University of Traditional Chinese Medicine Tianjin China
2. Department of Pharmacy Tianjin First Central Hospital Tianjin China
3. Mass Spectrometry Application Center Tianjin Key Laboratory of Medical Mass Spectrometry for Accurate Diagnosis Tianjin China
Abstract
AbstractIntroductionMenispermi Rhizoma (MR), the dried rhizome of Menispermum dauricum DC. (Menispermaceae), has been used to treat sore throat, enteritis, dysentery, and rheumatic arthralgia. Despite extensive research on its pharmacological effects, the chemical components in vitro and in vivo have not been thoroughly studied.ObjectiveTo establish an efficient method for rapid classification and identification of alkaloids in MR and its preparations, as well as metabolites in vivo after oral administration of MR.MethodsRapid identification of alkaloids and absorbed components of MR was performed using ultra‐performance liquid chromatography quadrupole time‐of‐flight mass spectrometry (UPLC‐Q‐TOF‐MS) coupled with UNIFI software. Moreover, the characteristic fragmentations and neutral losses of different types of alkaloids in MR were summarised to realise the rapid classification of alkaloids.ResultsA total of 55 components were unambiguously or tentatively identified in MR. Among them, 37 and 31 components were found in MR capsules and tablets, respectively. Meanwhile, 109 compounds were tentatively identified in rat plasma, urine and faeces, including 55 prototypes and 54 metabolites. Hydrogenation, hydroxylation, methylation, glucuronic acid and sulphate conjugations were the dominating metabolic fates of alkaloids.ConclusionThe data post‐processing strategy established could greatly enhance the structural identification efficiency. The results obtained might lay the foundation for further interpretation of clinical effects, mechanism of action and quality control of MR.
Funder
National Natural Science Foundation of China
Natural Science Foundation of Tianjin City
Subject
Complementary and alternative medicine,Drug Discovery,Plant Science,Molecular Medicine,General Medicine,Biochemistry,Food Science,Analytical Chemistry
Cited by
1 articles.
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