Immunological studies of β‐adrenergic receptors

Abstract

AbstractTwo types for antibodies have been raised against the β‐adrenergic receptor: either by injection of highly purified receptor from turkey erythrocytes or by injection of anticatecholamine ligand antibodies, and induction of anti‐idiotypic antibodies Our data illustrate the interactions of the β‐adrenergic receptor with these polyclonal antibodies. Preliminary results with monoclonal antibodies are also described. The redistribution of β‐receptors on intact cells is visualized by the use of fluorescent antibodies. Immunoprecipitation of radioiodinated receptor by the antireceptor antibodies yields a single major 60,000 MW component.