Affiliation:
1. Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College Beijing China
2. Yunnan International Joint Laboratory of Tropical Infectious Diseases & Key Laboratory of Insect‐borne Infectious Diseases Control in Yunnan Province of Yunnan Institute of Parasitic Diseases Yunnan China
3. Yunnan Institute of Parasitic Diseases Yunnan China
Abstract
AbstractMolecular surveillance is vital for monitoring arboviruses, often employing genus‐specific quantitative reverse‐transcription polymerase chain reaction (RT‐qPCR). Despite this, an overlooked chikungunya fever outbreak occurred in Yunnan province, China, in 2019 and false negatives are commonly encountered during alphaviruses screening practice, highlighting the need for improved detection methods. In this study, we developed an improved alphaviruses‐specific RT‐qPCR capable of detecting chikungunya virus, eastern equine encephalitis virus, western equine encephalitis virus, Venezuelan equine encephalitis virus, Sindbis virus, Mayaro virus, and Ross River virus with high sensitivity and specificity. The assay identified three chikungunya virus‐positive cases out of 188 sera retrospectively. Later genetic characterization suggested that imported cases from neighboring countries may be responsible for the neglected chikungunya fever outbreak of 2019 in Yunnan. Our findings underscore the value of improved alphaviruses‐specific RT‐qPCR in bolstering alphaviruses surveillance and informing preventive strategies.