Developmental rate displays effects of inheritance but not of sex in interpopulation hybrids of Tigriopus californicus

Abstract

AbstractCoevolved genetic interactions within populations can be disrupted by hybridization resulting in loss of fitness in hybrid individuals (i.e., hybrid breakdown). However, the extent to which variation in fitness‐related traits among hybrids is inherited across generations remains unclear, and variation in these traits may be sex‐specific in hybrids due to differential effects of genetic incompatibilities in females and males. Here we present two experiments investigating variation in developmental rate among reciprocal interpopulation hybrids of the intertidal copepod Tigriopus californicus. Developmental rate is a fitness‐related trait in this species that is affected by interactions between mitochondrial‐encoded and nuclear‐encoded genes in hybrids that result in variation in mitochondrial ATP synthesis capacities. First, we show that F2‐hybrid developmental rate is equivalent in two reciprocal crosses and is unaffected by sex, suggesting that breakdown of developmental rate is likely experienced equally by females and males. Second, we demonstrate that variation in developmental rate among F3 hybrids is heritable; times to copepodid metamorphosis of F4 offspring of fast‐developing F3 parents (12.25 ± 0.05 days, μ ± SEM) were significantly faster than those of F4 offspring of slow‐developing parents (14.58 ± 0.05 days). Third, we find that ATP synthesis rates in these F4 hybrids are unaffected by the developmental rates of their parents, but that mitochondria from females synthesize ATP at faster rates than mitochondria from males. Taken together, these results suggest that sex‐specific effects vary among fitness‐related traits in these hybrids, and that effects likely associated with hybrid breakdown display substantial inheritance across hybrid generations.