Effect of Ampelopsis brevipedunculata (Maxim.) Trautv extract on a model of atopic dermatitis in HaCaT cells and mice

Author:

Bak Seon Gyeong1ORCID,Lim Hyung Jin1ORCID,Won Yeong‐Seon1ORCID,Park Eun Jae1ORCID,Kim Young Hee2ORCID,Lee Seung Woong1ORCID,Oh Je Hun3ORCID,Kim Ji Eun3ORCID,Lee Min Jee3ORCID,Lee Soyoung1ORCID,Lee Seung Jae14ORCID,Rho Mun Chual1ORCID

Affiliation:

1. Functional Biomaterial Research Center Korea Research Institute of Bioscience and Biotechnology (KRIBB) Jeongeup South Korea

2. Division of Biotechnology and Advanced Institute of Environment and Bioscience College of Environmental and Bioresource Sciences, Jeonbuk National University Iksan South Korea

3. Ju Yeong NS Co., Ltd Seoul South Korea

4. Applied Biological Engineering, KRIBB School of Biotechnology University of Science and Technology Daejeon South Korea

Abstract

AbstractAmpelopsis brevipedunculata (Maxim.) Trautv. has been used for a long time as a folk remedy. According to studies, it possesses anti‐inflammatory, antioxidant, and antibacterial properties. However, its effects on atopic dermatitis (AD) are poorly studied. Thus, we investigated the therapeutic effect of A. brevipedunculata (Maxim.) Trautv. extract (ABE‐M) on 2,4‐dinitrochlorobenzene (DNCB)‐induced AD. For in vitro analysis, keratinocytes cell lines (HaCaT cells) were used. To evaluate the gene and protein expression levels of cytokines and chemokines, TNF‐α/IFN‐γ‐stimulated HaCaT cells were treated with ABE‐M. The cells and the supernatant were collected, then gene and protein levels were analyzed by real‐time polymerase chain reaction and enzyme‐linked immunosorbent assay analysis. For in vivo analysis, BALB/c mice (6 weeks) were randomly separated into five groups (n = 5). The mice were applied DNCB and phosphate‐buffered saline, dexamethasone (DX) or ABE‐M (50, 100, and 200 mg/kg) was orally administrated for 28 days. At the end, ear tissues and blood were collected for histological analysis and evaluation of cytokines and chemokines. In keratinocytes, ABE‐M inhibited the protein and mRNA levels of chemokines, and cytokines exposed by TNF‐α/IFN‐γ. Similarly, the expression of chemokines was suppressed by ABE‐M in AD animal model induced by DNCB and the level of pro‐inflammatory cytokines was decreased in a dose‐dependent manner. Our research indicates that ABE‐M could be a candidate material that can be used to improve skin immunity enhancement, health, and beauty.

Funder

Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries

Korea Research Institute of Bioscience and Biotechnology

National Research Council of Science and Technology

Publisher

Wiley

Subject

Food Science

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