Human fibroblast matrices bio-assembled under macromolecular crowding support stable propagation of human embryonic stem cells

Author:

Peng Yanxian1,Bocker Michael Thomas2,Holm Jennifer3,Toh Wei Seong,Hughes Christopher Stephen4,Kidwai Fahad5,Lajoie Gilles Andre4,Cao Tong5,Lyko Frank2,Raghunath Michael

Affiliation:

1. Department of Bioengineering, Faculty of Engineering; National University of Singapore; Singapore

2. Division of Epigenetics, DKFZ-ZMBH Alliance; German Cancer Research Center; Heidelberg; Germany

3. Department of Biomedical Engineering; Texas A&M University; College Station; Texas; 77843-3120; USA

4. Don Rix Protein Identification Facility, Department of Biochemistry, Schulich School of Medicine and Dentistry; University of Western Ontario; London; ON; Canada

5. Dental Research Lab, Discipline of Oral Sciences, Faculty of Dentistry; National University of Singapore; Singapore

Publisher

Wiley

Subject

Biomedical Engineering,Biomaterials,Medicine (miscellaneous)

Reference40 articles.

1. Pluripotent embryonal carcinoma clones derived from the human teratocarcinoma cell line Tera-2. Differentiation in vivo and in vitro;Andrews;Lab Invest,1984

2. Making sense of latent TGFβ activation;Annes;J Cell Sci,2003

3. Stable propagation of human embryonic and induced pluripotent stem cells on decellularized human substrates;Abraham;Biotechnol Prog,2010

4. Matrix revolutions: a trinity of defined substrates for long term expansion of human ESCs;Azarin;Cell Stem Cell,2010

5. Chromatin states of core pluripotency-associated genes in pluripotent, multipotent and differentiated cells;Barrand;Biochem Biophys Res Commun,2010

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