A Review of Reagents for Fluorescence Microscopy of Cellular Compartments and Structures, Part II: Reagents for Non‐Vesicular Organelles

Abstract

AbstractA wide range of fluorescent dyes and reagents exist for labeling organelles in live and fixed cells. Choosing between them can lead to confusion, and optimization for many of them can be challenging. Presented here is a discussion on the commercially available reagents that have shown the most promise for each organelle of interest, including endoplasmic reticulum/nuclear membrane, Golgi apparatus, mitochondria, nucleoli, and nuclei, with an emphasis on localization of these structures for microscopy. Included is a featured reagent for each structure with a recommended protocol, troubleshooting guide, and example image. © 2023 Wiley Periodicals LLC.Basic Protocol 1: Endoplasmic reticulum and nuclear membrane labeling using ER‐Tracker reagentsBasic Protocol 2: Labeling Golgi apparatus using dye‐labeled ceramidesBasic Protocol 3: Labeling mitochondria using MitoTracker Red CMXRosBasic Protocol 4: Labeling nucleoli using SYTO RNASelect Green