Affiliation:
1. School of Science, Chongqing University of Technology Chongqing China
2. School of Pharmacy and Bioengineering, Chongqing University of Technology Chongqing China
3. Chongqing Key Laboratory of Green Energy Materials Technology and Systems Chongqing China
Abstract
AbstractA single‐mode‐fiber (SMF)‐multimode‐fiber (MMF)‐tri‐core‐fiber (TCF) Michelson probe structure is proposed for trace detection of canine distemper virus (CDV). One end of the TCF is cut flat and fused with the multimode fiber, and the other end is coated with a silver film to enhance the reflection, and an optic‐fiber sensing probe with SMF‐MMF‐TCF structure is obtained. The (PDDA/PSS)3 multilayer film is modified on the surface of the fiber by layer‐by‐layer self‐assembly method as a polyelectrolyte binder to immobilize CDV antibodies to form a (PDDA/PSS)3/CDV antibody composite membrane for specific detection of CDV antigens. The response‐recovery test of the sensor is performed to verify its repeatability. The detection limit, the sensitivity, and the linear fitting degree for CDV antigen are 0.1236 pg/mL, 1.1776 dB/(pg/mL), and 0.9899, respectively. At the same time, the stability, selectivity, and clinical samples of the sensors were also verified.
Funder
Chongqing Municipal Science and Technology Bureau
Chongqing University of Technology
National Natural Science Foundation of China
Subject
General Physics and Astronomy,General Engineering,General Biochemistry, Genetics and Molecular Biology,General Materials Science,General Chemistry