Affiliation:
1. The United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province Mengchao Hepatobiliary Hospital of Fujian Medical University Fuzhou People's Republic of China
2. Mengchao Med‐X Center Fuzhou University Fuzhou People's Republic of China
3. College of Biological Science and Engineering Fuzhou University Fuzhou People's Republic of China
4. Liver Disease Center The First Affiliated Hospital of Fujian Medical University Fuzhou People's Republic of China
5. CAS Key Laboratory of Design and Assembly of Functional Nanostructures Fujian Institute of Research on the Structure of Matter Chinese Academy of Sciences Fuzhou People's Republic of China
Abstract
AbstractThe immune status of tumor‐infiltrating lymphocytes (TILs) is essential for the effectiveness of cancer immunotherapies. However, due to the diversity of immune status in TILs, cellular heterogeneity, and the applicability to the clinic, it is still lacking effective strategies to meet clinical needs. We developed a novel immuno‐recognition‐induced method based on rolling circle amplification (RCA), namely immunoRCA, to in situ visualize the immune status of TILs in actual clinical samples. This developed immunoRCA method, in which, feature mRNAs were used as the biomarkers for the immune status of TILs, has a low fluorescence background, high sensitivity, and specificity. The immunoRCA was able to efficiently evaluate the immune status of CD8+ T cells regulated by activating or inhibiting factors, track the T cell type and immune status during in vitro expansion, and in situ visualize the number, location, and immune status of TILs in clinical specimens.
Funder
China Postdoctoral Science Foundation
National Natural Science Foundation of China
Natural Science Foundation of Fujian Province
Subject
General Physics and Astronomy,General Engineering,General Biochemistry, Genetics and Molecular Biology,General Materials Science,General Chemistry