Affiliation:
1. Post Graduate Program in Medicine–Biophotonic, University Nove de Julho (UNINOVE) São Paulo Brazil
2. Postgraduate Program in Medicine, Universidade Nove de Julho, UNINOVE São Paulo Brazil
3. Universidad de Vic–Universidade Central de Cataluña, UVic Vic Spain
4. Translational Medicine, Federal University of São Paulo‐UNIFESP São José dos Campos Brazil
Abstract
AbstractThe objective of the study was to evaluate the effect of photobiomodulation (PBM) with laser on the inflammatory process in an experimental in vitro model of ACO. The groups were: (1) human bronchial epithelial cells (BEAS‐2B); (2) BEAS‐2B cells treated with dexamethasone; (3) BEAS‐2B cells irradiated with laser; (4) BEAS‐2B cells stimulated with cigarette smoke extract (CSE) + House Dust Mite (HDM); (5) BEAS‐2B cells stimulated with CSE + HDM and treated with dexamethasone; (6) BEAS‐2B cells incubated with CSE + HDM and irradiated with laser. After 24 h, cytokines were quantified. There was a reduction in TNF‐α, IL‐1β, IL‐6, IL‐4, IL‐5, IL‐13, IL‐17, IL‐21, IL‐23, and an increase in IL‐10 and IFN‐γ in cells from the laser‐irradiated ACO group compared to only ACO group. With these results, we can suggest that photobiomodulation acts in the modulation of inflammation observed in ACO, and may be a treatment option.
Funder
Fundação de Amparo à Pesquisa do Estado de São Paulo
Universidade Nove de Julho