Label‐free assessment of pathological changes in pancreatic intraepithelial neoplasia by biomedical multiphoton microscopy
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Published:2024-01-14
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Volume:
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ISSN:1864-063X
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Container-title:Journal of Biophotonics
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language:en
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Short-container-title:Journal of Biophotonics
Author:
Miao Jikui1,
Zhang Zheng1,
Zhang Xiong1,
Huang Xingxin1,
Zhang Shichao1,
Zhan Zhenlin1,
Chen Jianxin1ORCID,
Chen Linying2,
Li Lianhuang1ORCID
Affiliation:
1. Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory of Photonics Technology, College of Photonic and Electronic Engineering Fujian Normal University Fuzhou China
2. Department of Pathology The First Affiliated Hospital of Fujian Medical University Fuzhou China
Abstract
AbstractPancreatic intraepithelial neoplasia (PanIN) is the most common precursor lesion that has the potential to progress to invasive pancreatic cancer, and early and rapid detection may offer patients a chance for treatment before the development of invasive carcinoma. Therefore, the identification of PanIN holds significant clinical importance. In this study, we first used multiphoton microscopy (MPM) combining two‐photon excitation fluorescence and second‐harmonic generation imaging to label‐free detect PanIN and attempted to differentiate between normal pancreatic ducts and different grades of PanIN. Then, we also developed an automatic image processing strategy to extract eight morphological features of collagen fibers from MPM images to quantify the changes in collagen fibers surrounding the ducts. Experimental results demonstrate that the combination of MPM and quantitative information can accurately identify normal pancreatic ducts and different grades of PanIN. This study may contribute to the rapid diagnosis of pancreatic diseases and may lay the foundation for further clinical application of MPM.
Funder
National Natural Science Foundation of China
Natural Science Foundation of Fujian Province
Subject
General Physics and Astronomy,General Engineering,General Biochemistry, Genetics and Molecular Biology,General Materials Science,General Chemistry